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目的 研究银杏叶提取物 (EGB)的胃粘膜保护作用及其机制。方法 采用大鼠RCS和ig 30 %乙醇、小鼠ig无水乙醇和im吲哚美辛诱发的AGML模型。结果 ①EGB(2 5、50、10 0mg·kg- 1,ig× 5d)可以明显降低上述几种AGML模型的胃粘膜损伤指数 ,且呈一定的量效依赖关系。②EGB对胃液分泌量、胃液酸度和胃蛋白酶活性无明显影响。③EGB可以明显增加大鼠胃壁结合粘液的分泌量。④EGB可以明显降低血清和胃粘膜中升高的MDA含量 ,提高SOD活性。⑤EGB可以升高血清和胃粘膜中PGE2 和NO含量 ;同时EGB预处理可明显拮抗L NNA的加重损伤作用。⑥EGB可以提高西米替丁的抗胃损伤作用。结论 ①EGB可能通过清除自由基及增强内源性抗氧化系统而保护胃粘膜。②EGB的胃粘膜保护作用可能与内源性PGs和NO有关。③EGB与西米替丁合用可产生协同作用 ,为今后联合用药治疗消化性疾病提供了实验依据。
Objective To study the gastric mucosal protective effect of Ginkgo biloba extract (EGB) and its mechanism. Methods The AGML model induced by rat RCS and ig 30 % ethanol, mouse ig anhydrous ethanol and imidoxine was used. Results 1EGB (25, 50, 100 mg·kg-1, ig×5d) could significantly reduce the gastric mucosal injury index of the above-mentioned several AGML models, and showed a certain dose-effect dependence. 2EGB had no significant effect on gastric juice secretion, gastric juice acidity and pepsin activity. 3EGB can significantly increase the secretion of mucin in the gastric wall of rats. 4EGB can significantly reduce elevated MDA levels in serum and gastric mucosa and increase SOD activity. 5EGB can increase PGE2 and NO content in serum and gastric mucosa, while EGB pretreatment can significantly antagonize the aggravating injury effect of LNNA. 6EGB can improve the anti-gastric effects of cimetidine. Conclusion 1EGB may protect gastric mucosa by scavenging free radicals and enhancing endogenous antioxidant system. The gastric mucosal protective effect of 2EGB may be related to endogenous PGs and NO. The combination of 3EGB and cimitidine can produce a synergistic effect, providing an experimental basis for the future combination therapy for the treatment of digestive diseases.