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目的:探讨葡萄糖酸铬对体外高糖培养乳鼠心肌细胞的保护作用及其机制。方法:Wistar乳鼠原代细胞随机分为空白对照组、高糖组及葡萄糖酸铬高、低剂量组。用荧光显微镜测定各组细胞凋亡情况。结果:经葡萄糖酸铬干预后,心肌细胞存活率明显增高,凋亡细胞明显减少,bcl-2表达增加,caspase-3表达减少。结论:葡萄糖酸铬抑制体外高糖培养的心肌细胞凋亡,增高细胞存活率,其机制可能与抑制caspase-3、激活bcl-2表达有关。
Objective: To investigate the protective effect of chromium gluconate on neonatal rat cardiomyocytes cultured in high glucose and its mechanism. Methods: Primary cells of Wistar suckling mice were randomly divided into blank control group, high glucose group and chromium gluconate high and low dose groups. Fluorescence microscopy was used to determine the apoptosis of each group. Results: After the intervention of chromium gluconate, the survival rate of cardiomyocytes was significantly increased, the number of apoptotic cells was significantly decreased, the expression of bcl-2 was increased and the expression of caspase-3 was decreased. CONCLUSION: Chromium gluconate can inhibit cardiomyocyte apoptosis induced by high glucose in vitro and increase cell viability. The mechanism may be related to the inhibition of caspase-3 and the activation of bcl-2.