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用控制磷脂及其代谢来源的半合成低硒低磷脂基础饲料和分别补硒、补磷脂、双补及补足磷脂代谢因子的全补饲料喂养豚鼠3个月后,用紫外分光光度计联用自动循环水浴对动物心肌细胞色素氧化酶活性作了反应动力学研究。结果表明,基础饲料喂养的动物心肌细胞色素氧化酶活性最低。单纯补硒,补磷脂虽稍有改善,但差别不显著,只有同时补充的尤其再补足与内源性磷脂代谢有关的因子(胆碱及蛋氨酸)时其速度常数K才显著高于基础饲料组,甚至超过常备饲料组,同时心肌磷脂的组成尤其作为细胞色素氧化酶的特异结合脂质的心磷脂(Cardiolipin)量也均达到了常备饲料组的水平。文中讨论了膜结合酶的结合性脂质(界面脂)对酶活性的重要意义和克山病的可能病因及发病学。
With the control of phospholipid and its metabolic origin of semi-synthetic low-selenium low-phospholipid basal diet and supplementation of selenium, lecithin, double-supplement and make up the full complement of phospholipid metabolic factors fed guinea pigs after 3 months, with UV spectrophotometer with automatic Kinetic study on the activity of cardiomyocyte cytochrome oxidase in a circulating water bath. The results showed that basal diet fed animal myocardial cytochrome oxidase activity of the lowest. Pure selenium supplementation, although a slight improvement in phospholipid, but the difference was not significant, only at the same time complement, especially to complement the endogenous phospholipid metabolism-related factors (choline and methionine) when the rate constant K was significantly higher than the basic diet group , Even more than the normal feed group. Meanwhile, the amounts of myocardial phospholipid and cardiolipin, which are the specific binding lipids of cytochrome oxidase, also reached the level of the conventional feed group. This article discusses the membrane-bound enzyme lipid (interface lipid) on the enzyme activity of significance and Keshan disease possible etiology and pathogenesis.