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观察空间诱变后恶性黑色素瘤B16细胞株的在小鼠体内肿瘤组织中血管生成拟态数目的改变,并探讨可能的机理。应用细胞低温长期生存系统,将B16细胞株搭载于中国第20号返回式卫星,返地后单克隆化,从得到的110株单克隆空间诱变B16细胞株中随机选取7株,编号为38#～44#,常规培养6代后和对照细胞株在荧光倒置显微镜下观察肿瘤细胞的形态;同时采用MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,又称噻唑蓝)法,检测细胞增殖情况,将增殖差异较大的39#和44#细胞株接皮下种于C57BL/6J小鼠,两周后颈椎脱臼法处死小鼠,取出瘤体,经福尔马林固定后,采用CD31和PAS套染的方法观测肿瘤组织血管生成拟态的情况,并推测其与肿瘤生长速度和转移可能关系。
To observe the change of mimicry number of angiogenesis in malignant melanoma B16 cell line in mice after space mutagenesis and to explore the possible mechanism. The B16 cell line was mounted on a Chinese returning satellite No. 20 using the cryogenic long-term cell survival system. After returning to the ground, the B16 cell line was cloned. Seven randomly selected strains of B16 cell line were obtained from 110 strains of monoclone space-mutated # ~ 44 #. After 6 generations of conventional culture, the morphology of the tumor cells was observed under a fluorescence inverted microscope. MTT (3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide C57BL / 6J mice were inoculated subcutaneously in 39 # and 44 # cell lines with large differences in proliferation. After two weeks, mice were sacrificed by cervical dislocation and the tumor was removed. After formalin fixation, the tumor mimicry of angiogenesis was observed by CD31 and PAS staining, and the relationship between the mimicry and the tumor growth rate and metastasis was estimated.