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目的观察中药制剂心悸宁丸对肾上腺素致室性早搏大鼠模型心律、血清NO和ET-1水平的影响,探讨该药物预防室性早搏的可能作用机制。方法将48只健康雄性SD大鼠随机分为6组,每组8只,即:健康组、心悸宁丸低剂量组(0.487 g/kg)、心悸宁丸中剂量组(0.974g/kg)、心悸宁丸高剂量组(1.948g/kg)、普萘洛尔组(2mg/kg)和模型组。各组大鼠每日清晨空服灌胃给药1次,每次2ml。其中健康组和模型组每日给予同等剂量的生理盐水灌胃,连续10天。第10天末次给药1小时后,除健康组外,其余五组以鼠尾静脉快速注射盐酸肾上腺素(100μg/kg)诱发大鼠心律失常模型,记录心律失常出现时间、心律失常持续时间和大鼠心率的变化,统计各组大鼠室性早搏发生率,最后从腹腔动脉采血测定血清NO和ET-1的含量。结果与模型组相比,心悸宁丸高、中剂量组和普萘洛尔组在推迟心律失常出现时间和缩短心律失常持续时间方面作用明显,有显著性差异(P<0.05);与模型组相比,心悸宁丸高、中、低各剂量组均能降低大鼠室早发生率;与模型组相比,心悸宁丸高、中、低各剂量组和普萘洛尔组均能使大鼠血清NO含量明显增加,ET-1含量明显减少,差异显著有统计学意义(P<0.05)。结论心悸宁丸能较好的对抗由肾上腺素诱发的大鼠心律失常,使心律失常出现时间延迟,心律失常持续时间缩短并显著低室性早搏发生率,且与剂量大小呈正相关,中药高剂量组的拮抗作用最好;心悸宁丸能明显提高室性早搏大鼠血清NO的水平,显著降低血清ET-1的水平,保护血管内皮,对室性早搏具有良好的预防作用。
Objective To observe the effect of Chinese medicine preparation Palintinning pill on cardiac arrhythmia, serum NO and ET-1 levels in rats with adrenaline-induced ventricular premature beat and to explore its possible mechanism of action in preventing premature ventricular contractions. Methods Forty-eight healthy male Sprague-Dawley rats were randomly divided into 6 groups with 8 rats in each group: healthy group, low dose of palmatine pill (0.487 g / kg), middle dose of palpitating pill (0.974 g / kg) , High-dose palpitation-ningwan group (1.948g / kg), propranolol group (2mg / kg) and model group. The rats in each group were given oral gavage once a day for 2ml each time. The healthy group and model group were given the same dose of saline daily gavage for 10 consecutive days. One hour after the last administration on the 10th day, except the healthy group, the other five groups were induced by rapid injection of epinephrine hydrochloride (100μg / kg) into the caudal vein to induce the rat arrhythmia model. The arrhythmia occurrence time, arrhythmia duration, The changes of heart rate in rats were observed. The incidence of ventricular premature beats in each group was calculated. The content of serum NO and ET-1 in the serum was measured from the celiac artery. Results Compared with the model group, the effects of high-dose, middle-dose and propranolol groups were significant (P <0.05) in retarding the occurrence of arrhythmia and shortening the duration of arrhythmia. Compared with model group Compared with the model group, the high, middle and low dose groups of palpitations and the propranolol groups were able to make The content of NO in rat serum increased significantly, the content of ET-1 decreased significantly, the difference was statistically significant (P <0.05). Conclusion Pinyin pill can better antagonize the adrenaline-induced arrhythmia in rats, arrhythmia delay time, the duration of arrhythmia shortened significantly and the incidence of premature ventricular contractions, and the dose size was positively correlated with high dose of traditional Chinese medicine The best antagonistic effect was observed in rats. Xinyanxin Tablet could obviously improve the level of serum NO in ventricular premature rats, significantly lower the level of ET-1, protect the vascular endothelium and prevent ventricular premature beats.