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目的观察转染微小RNA-132(miR-132)对体外人骨肉瘤细胞株SaOS-2生长和凋亡的影响，并初步探讨其作用机制。方法实时荧光定量逆转录-聚合酶链反应（RT-qPCR）检测34例骨肉瘤组织及癌旁正常组织中miR-132 mRNA的表达；Cel Counting Kit-8(CCK-8)法检测细胞增殖；细胞经DAPI染色后在荧光显微镜下观察细胞凋亡；Western blot检测体外SaOS-2细胞中Mucin-4、HER-2和p-FAK蛋白的表达。结果 miR-132 mRNA在骨肉瘤组织中的表达明显低于癌旁正常组织，差异有统计学意义(<0.05)；与对照组相比较，转染组细胞中miR-132 mRNA的表达明显升高，细胞増殖被明显抑制，凋亡细胞显著增加，差异均有统计学意义（<0.05）；Mucin-4、HER-2和p-FAK蛋白在转染组细胞中的表达均下调，与对照组相比较，均有统计学差异（<0.05）。结论转染miR-132对体外骨肉瘤细胞有增殖抑制和凋亡诱导作用，miR-132有望成为骨肉瘤治疗的新靶点。“,”Objective To observe the biological role and underlying mechanism of miR-132 in osteosarcoma proliferation and apoptosis. Methods The expression of miR-132 mRNA in the cancer tissue and their adjacent tissues in 34 osteosarcoma patients were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The biological ef ects of miR-132 transfection on osteosarcoma SaOS-2 cel s were assessed by CCK-8 assay and DAPI staining. Western blot ing was used to detect the expression of Mucin-4, HER-2 and p-FAK in SaOS-2 cel s. Results The expression level of miR-132 mRNA was found to be downregulated in osteosarcoma tissues compared with the matched adjacent tissues. After transfection, the expression of miR-132 mRNA was significantly higher than control group. The proliferation of SaOS-2 cel s was inhibited significantly by miR-132 transfection. Apoptosis rate induced by the miR-132 transfection was markedly higher than that of control. The proteins of Mucin-4, HER-2 and p-FAK were decreased after miR-132 transfection. Conclusion miR-132 is downregulated in osteosarcoma tissues, transfected of miR-132 can ef ectively inhibit proliferation and promote apoptosis of SaOS-2 cel s in vitro, miR-132 may become a new target for the regulation of gene expression in osteosarcoma.