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首先利用鱼类线粒体COⅠ基因两端的保守序列设计简并引物,扩增测序获得了细鳞鲴COⅠ基因1 551 bp序列全长。然后利用COⅠ基因部分序列标记对千岛湖汾口(FK)、姜家镇(JJZ)、富文(FW)以及临岐(LQ)4个细鳞鲴群体84个样本的遗传多样性和遗传结构进行了分析,结果表明:在4个群体中共检测到4种不同单倍型。四群体的单倍型多样性(h)分别为0.500 0、0、0.142 5和0.128 7,核苷酸多样性(π)分别为0.001 9、0、0.000 2和0.000 2,其中FK群体的单倍型多样性(h)和核苷酸多样性(π)水平最高,另外3个群体单倍型多样性(h)和核苷酸多样性(π)水平都较低。AMOVA分析显示,群体间遗传变异占总变异的23.56%,群体内的遗传变异占总变异的76.44%,表明群体内变异是总变异的主要来源。研究结果表明千岛湖4个群体间没有明显的遗传分化。
Firstly, the degenerate primers were designed based on the conserved sequences of mitochondrial COⅠ gene in fish. The full-length cDNA of COⅠ gene was obtained by amplification and sequencing. Then, the genetic diversity and genetic structure of 84 samples from 4 populations of Fagaceae (FK), JJZ, FW (FW) and Linqi (LQ) The results showed that four different haplotypes were detected in four populations. The haplotype diversity (h) of the four populations were 0.500 0,0,0.142 5 and 0.128 7, and the nucleotide diversity (π) was 0.001 9,0,0.000 2 and 0.000 2, respectively The haplotype diversity (h) and nucleotide diversity (π) were the highest, while the other three populations had lower haplotype diversity (h) and nucleotide diversity (π). AMOVA analysis showed that the genetic variation among populations accounted for 23.56% of the total variation, and the genetic variation within the population accounted for 76.44% of the total variation, indicating that variation within the population is the main source of total variation. The results show that there is no obvious genetic differentiation among the four groups in Qiandao Lake.