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目的探讨rSj26-Sj32-Immunogold-IgG-Dot-ELISA用于慢性日本血吸虫病的诊断价值。方法分别以rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)作为包被抗原,通过Immunogold-IgG-Dot-ELISA检测慢性日本血吸虫病患者血清抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎、肺结核病人血清及健康人血清作为对照。结果 rSj26-Sj32-Immunogold-IgG-Dot-ELISA检测日本血吸虫抗体的敏感性和特异性分别为95.00%和97.67%,且与华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎和肺结核患者血清均无交叉反应;诊断慢性日本血吸虫病的阳性预告值、阴性预告值分别为97.44%和95.45%,诊断效率为96.39%,与SjAWA-Immunogold-IgG诊断效率(96.39%)相同。结论 rSj26-Sj32-Immunogold-IgG-Dot-ELISA法可用于慢性日本血吸虫病的诊断。
Objective To investigate the diagnostic value of rSj26-Sj32-Immunogold-IgG-Dot-ELISA for chronic schistosomiasis japonica. Methods The serum antigens of patients with chronic schistosomiasis japonica were detected by immunogold-IgG-Dot-ELISA using rSj26-Sj32 fusion protein and SjAWA as coating antigen, respectively. Meanwhile, clonorchiasis Trematodes, alveolar echinococcosis, cystic echinococcosis, hepatitis B, tuberculosis patients serum and healthy human serum as a control. Results The sensitivity and specificity of rSj26-Sj32-Immunogold-IgG-Dot-ELISA for the detection of Schistosoma japonicum antibody were 95.00% and 97.67%, respectively, and were associated with Clonorchiasis sinensis, Paragonimus westermani, The positive predictive value and negative predictive value of diagnosing chronic schistosomiasis japonica were 97.44% and 95.45% respectively, and the diagnostic efficiency was 96.39%, which was consistent with that of SjAWA -Immunogold-IgG diagnostic efficiency (96.39%) the same. Conclusion rSj26-Sj32-Immunogold-IgG-Dot-ELISA can be used to diagnose chronic schistosomiasis japonica.