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用大白菜、荞麦、多花菜豆、薏苡和芝麻等五种作物,每作物4个品种,各设25、50、100、150、200株不同群体的田间种植试验。采用纱网隔离,人工链式杂交,成对杂交,混合授粉,人工辅助授粉或虫媒(蜜蜂、苍蝇)等不同传粉方法。观察、记载主要农艺性状。室内考种并测定各繁种方法与原种繁殖前后10种同工酶,计算遗传多样性指数;比较不同种群及原种与不同繁种方法繁殖后代间的差异显著性(遗传相似性)。提出这5种作物基因库种子最佳繁种措施:大白菜种植50～100株,群体内进行人工链式杂交;荞麦在网罩内种50～100株,采用家蝇传粉;多花菜豆在全封闭网棚内种50株以上,放蜜蜂传粉;薏苡种50～150株群体,套袋人工混合授粉;芝麻采用非隔离区种植40～50株或利用温室隔离种植。
Five Chinese cabbage, buckwheat, flowering bean, Coix and sesame were used to test the field cultivation of four varieties per plant and 25,50,100,150,200 different groups. Using gauze isolation, artificial chain hybridization, pair crossing, mixed pollination, artificial pollination or insect media (bees, flies) and other pollination methods. Observation, record the main agronomic traits. Indoor test species and determination of the various breeding methods and the original species before and after breeding of 10 isozymes, genetic diversity index calculation; comparison of different populations and different species of breeding methods and breeding progenies significant difference (genetic similarity). The best breeding measures for these five kinds of crop genebanks were proposed: 50-100 cabbage plants were planted, and artificial chain hybridization was carried out in the population; 50-100 plants of buckwheat were planted in the grille and pollinated by housefly; Fully enclosed net shed more than 50 species, put bee pollination; Coix 50-150 groups, bagging artificial mixed pollination; sesame non-isolated area with 40 to 50 plants or greenhouse cultivation.