论文部分内容阅读
目的：观察 Er：YAG激光与根管冲洗剂联合应用对根尖外多细菌生物膜的杀菌作用。方法：体外牛牙骨质片上构建根尖外多细菌（衣氏放线菌、血链球菌和粪肠球菌）生物膜并随机分为6组。分别按以下方法进行处理，A组：52．5 g／L次氯酸钠（NaClO）液＋Er：YAG激光；B组：5 g／L NaClO液＋Er：YAG激光；C组：20 g／L洗必泰（CHX）液＋Er：YAG激光；D组：酸性水（SAEW）＋Er：YAG激光；E组：生理盐水（阴性对照组）；F组：52．5 g／L NaClO液（阳性对照组）。每组又分为3个亚组，分别处理30 s、1 min、3 min（n＝6）。SEM观察处理后多细菌生物膜形态，实时荧光定量PCR检测各组间细菌数量变化。结果：各实验组的细菌数量在各时间点均显著低于阴性对照组（P＜0．05）。其中A组杀菌效果最强，在30 s和3 min时与F组相比无统计学差异（P＞0．05），1 min时杀菌效果优于F组（P＜0．05）。B、D组在1、3 min时与F组相比无统计学差异（P＞0．05），30 s杀菌效果较F组略差（P＜0．05）。C组的杀菌效果在各时间点均弱于F组（P＜0．05）。结论：Er：YAG激光与几种根管冲洗剂联合应用能杀灭根尖外微生物。“,”AIM:To evaluate the bactericidal effective of the Er:YAG laser combined with different irrigants on apical multispecies biofilm.METHODS:Actinomyces,Enterococcus faecalis and Streptococcus sanguis were co-cultured on bovine cementum slices to form multispecies biofilm.The specimens were divided into 6 groups randomly and were treated as the following:52.5 g/L sodium hypochlorite (NaClO)+Er:YAG laser (group A),5 g/L NaClO+Er:YAG laser (group B),20 g/L chlorhexidine (CHX)+Er:YAG laser (group C),strong acid electrolytic water (SAEW)+Er:YAG laser (group D),normal saline (group E,negative control),and 52.5 g/L NaClO (group F, positive control).Each group was divided into 3 subgroups(n=6).In each subgroup,the specimens were treated with different methods for 30 s,1 min and 3 min respectively.Scanning electron microscope(SEM)was used to observe the morphology of multispecies biofilm after treatment.Real-time quantitative PCR was used to detect the bacterial num-ber.RESULTS:The number of bacteria in every treatment group was significantly less than that in the negative control group(P0.05)and stronger in 1 min(P0.05),but showed less effect in 30s(P<0.05).The antibacterialeffect of group C is poorer compared with other groups except negative group at all time points(P<0.05).CONCLU-SION:Er:YAG combined with different root canal irrigants can remove the apical multispecies biofilm effectively.