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水稻离体茎穗室内杂交技术是广东省农科院最先创造的。他们把从田间取回的茎穗(不带根)在室内杂交后,将母本茎穗插植在人工配制的培养液中,置于有散射光的房间内让其发育结实直至成熟。贵州农学院吴平理等对此方法进行了改进,将母本离体茎穗不带根人工培养液栽培改为离体茎穗带根泥土栽培。我们在此基础上改进了父本处理技术,解决了父母本花时不遇的问题,又大幅度地提高了杂交工效和杂交质量,使这一方法日趋完善。此方法有如下优点: 1.工效高。用此种方法进行水稻杂交,一般1人1天可做30~60个母本穗,如用不育系作母本,1人1天可做100~200个母本穗,比普通的田
In vitro hybridization of stems and leaves of rice is the first creation of Guangdong Provincial Academy of Agricultural Sciences. After they got the stem spikes (without roots) retrieved from the field and crossed them in the house, they stabbed the spikelets of the female parent in a man-made medium and placed them in a room with scattered light to allow them to grow and mature until they matured. Guizhou Academy of Agricultural Sciences, such as Wu Ping-li, this method has been improved, the female parent stem tissue culture without root culture instead of stems and roots with root soil culture. Based on this, we improved the paternal handling technology to solve the problems that parents never had when they spent their time and greatly improved the efficiency of hybridization and the quality of hybridization. This method is getting more and more perfect. This method has the following advantages: 1. High efficiency. In this method for rice hybrids, usually 1 person a day can do 30 to 60 female spikes, such as male sterile lines as a female parent, 1 person can do 100 ~ 200 female parent a day, than ordinary fields