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用高效液相色谱法分离测定大黄中的蒽醌类化合物,已有报道。作者选用内加法应用高效液相色谱仪测定大黄中五种蒽甙的含量。以甲醇—水—甲酸作梯度洗脱,并利用样品中的一个峰作为参比,得到满意结果。
Separation and determination of terpenoids in rhubarb by high performance liquid chromatography have been reported. The authors used the internal additive method for the determination of five kinds of rhenium in rhubarb using high performance liquid chromatography. Methanol-water-formic acid was used as a gradient elution, and one of the peaks in the sample was used as a reference to obtain satisfactory results.