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用间接免疫荧光流式细胞术测定阵发性睡眠性血红蛋白尿症（ＰＮＨ）患者和正常人红细胞乙酰胆碱酯酶（ＡｃｈＥ）表达量，发现ＰＮＨ的ＡｃｈＥ表达远远低于正常，而且蛇毒溶血试验与ＡｃｈＥ的缺失呈正相关。由于ＰＮＨ正常和异常血细胞同时并存，为进一步研究ＰＮＨ的ＡｃｈＥ（－）红细胞，采用ＰｒｏｔｅｉｎＡＳｅｐｈａｒｏｓｅ６ＭＢ结合ＡｃｈＥ单抗亲和层析法分离出ＰＮＨ的ＡｃｈＥ（－）红细胞。用￣３Ｈ－肌醇标记实验证明，正常红细胞膜在区带４．１有较高放射活性，而ＰＮＨ红细胞膜放射活性极低，ＰＮＨ的ＡｃｈＥ（－）红细胞膜完全无放射活性；用免疫印渍实验证明区带４．１是ＡｃｈＥ存在部位。用酶联免疫方法直接检测ＰＮＨ患者和正常人血浆中ＡｃｈＥ含量，结果发现ＰＮＨ患者血浆中有ＡｃｈＥ存在，而正常人未能检出。
Indirect immunofluorescence flow cytometry in patients with paroxysmal nocturnal hemoglobinuria (PNH) and normal erythrocyte acetylcholinesterase (AchE) expression levels and found that PNH AchE expression is much lower than normal, and venom hemolysis test and AchE loss was positively correlated. PNH AchE (-) erythrocytes were isolated by ProteinASepharose6MB combined with AchE monoclonal antibody affinity chromatography for further study of AchE (-) erythrocytes of PNH due to coexistence of normal and abnormal PNH blood cells. 3H-myo-inositol labeling experiments showed that the normal erythrocyte membrane in zone 4.1 has a higher radioactivity, while the PNH erythrocyte membrane activity was very low, PNH AchE (-) erythrocyte membrane completely radioactive; immunofluorescence Staining experiments show zone 4.1 is the presence of AchE site. Enzyme-linked immunosorbent assay was used to directly detect plasma AchE levels in patients with PNH and in normal subjects. The results showed that there was AchE in the plasma of patients with PNH, but not in normal subjects.