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目的观察凹顶藻萜类化合物(Laurencia terpenoids extract,LTE)对酒精暴露大鼠的抗氧化水平及HO-1酶活性的影响,并探讨其可能的作用机制。方法60只雄性Wistar大鼠随机分为6组。酒精模型组(B组)给予乙醇4.8g/kgbw·d灌胃;LTE低、中、高剂量干预组(C、D、E组)分别给予LTE25、50、100mg/kgbw·d;甘利欣药物组(F组)给予甘利欣200mg/kgbw·d灌胃。空白对照组(A组)给予等体积蒸馏水。除空白组外,酒精剂量均同模型组。实验进行6w。分别测定血清及肝匀浆中超氧化物歧化酶(SOD)和谷胱甘肽过氧物酶(GSH-Px)活性,丙二醛(MDA)含量。对肝组织切片进行免疫组化染色,观察肝内血红素氧化酶-1(HO-1)免疫阳性细胞的组织分布,并对其灰度值和光吸收密度值进行定量分析。结果与A组比,B、F组血清及肝匀浆SOD活力下降,C、E组血清SOD活力比B组升高,有一定的量效关系。B组大鼠血清及肝匀浆GSH-Px活力较A组下降,而D组较B组显著升高。与A组比,B组血清及肝匀浆MDA含量较A组升高,而D、E组血清和肝匀浆MDA含量较B组显著降低,且有一定的量效关系。与A组比,B组HO-1活力显著降低。D、E、F组HO-1活力比B组升高明显。结论LTE可增强酒精暴露大鼠体内抗氧化的活性,减少脂质过氧化产物的生成,诱导HO-1活性增强,从而对酒精造成的机体氧化损伤表现出相应的保护效应。
Objective To observe the effects of Laurencia terpenoids extract (LTE) on the antioxidant status and the activity of HO-1 in alcohol-exposed rats and to explore its possible mechanism. Methods Sixty male Wistar rats were randomly divided into 6 groups. The alcohol group (group B) was given intragastric administration of ethanol (4.8g / kg bw · d); the low, medium and high dose intervention groups of LTE (group C, D and E) Group (F group) were given Gan Lixin 200mg / kgbw · d gavage. The blank control group (group A) was given equal volume of distilled water. Except for the blank group, the alcohol dose was the same as the model group. Experiment for 6w. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in serum and liver homogenate were measured respectively. Immunohistochemical staining of liver tissue sections was performed to observe the tissue distribution of heme oxygenase-1 (HO-1) immunoreactive cells in the liver and the quantitative analysis of the gray value and light absorption density. Results Compared with group A, the activity of SOD in serum and liver homogenate decreased in groups B and F, and the activity of SOD in groups C and E was higher than that in group B, and there was a certain dose-effect relationship. The GSH-Px activity in serum and liver homogenate of group B decreased compared with that of group A, while the level of GSH-Px in group B was significantly higher than that of group B. Compared with group A, the content of MDA in serum and liver homogenate of group B was higher than that of group A, while the content of MDA in serum and liver homogenate of group D and group E was significantly lower than that of group B, and had a certain dose-effect relationship. Compared with group A, HO-1 activity in group B decreased significantly. The activity of HO-1 in group D, E, F was higher than group B obviously. CONCLUSION: LTE can enhance the anti-oxidative activity, reduce the production of lipid peroxidation products and increase the activity of HO-1 in alcohol-exposed rats, and thus protect the body against oxidative damage induced by alcohol.