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植物病程相关(PR)基因一般在病原物侵染过程中受诱导发生转录上调.目前有证据提示植物PR基因在非生物逆境胁迫下也发生转录变化,但其蛋白质的表达变化情况还鲜有报道.为了解水稻PR蛋白质在逆境胁迫下的表达特征,本文采用免疫印迹技术(Western blotting,WB)调查了8个PR蛋白质在冷、热、旱、淹和盐等5种胁迫下的表达谱.结果表明:在冷胁迫下PR8表达上调,在热胁迫下PR1a、PR3、PR5和PR16表达下调;在旱胁迫下PR1a、PR2和PR8表达上调,而PR5和PR16表达下调,在淹胁迫下PR1、PR2和PR15表达上调,PR1a、PR3、PR5和PR8表达下调;在盐胁迫下PR2和PR3表达上调,而PR1a、PR5、PR8和PR16表达下调.另外,对这些PR基因的上游启动子区进行分析,发现存在与胁迫响应相关的调控元件,其中脱落酸反应元件(ABRE)、TC-rich repeats和HSE的出现频率较高.这些蛋白质表达数据进一步佐证了PR蛋白在逆境胁迫反应中发挥着重要且不尽相同的作用.
Plant pathology-related (PR) genes are generally induced to undergo transcriptional upregulation during pathogen infestation.At present, there is evidence that the plant PR gene also undergoes transcriptional changes under abiotic stress conditions, but the changes of its protein expression are seldom reported In order to understand the expression characteristics of rice PR under stress stress, we used Western blotting (WB) to investigate the expression profiles of eight PR proteins under the stress of cold, heat, drought, flooding and salt stress. PR1a, PR3, PR5 and PR16 were down-regulated under heat stress. Under drought stress, the expression of PR1a, PR2 and PR8 were up-regulated while the expression of PR5 and PR16 were down-regulated. Under the stress of flooding, PR2 and PR15 were up-regulated, PR1a, PR3, PR5 and PR8 were down-regulated, PR2 and PR3 were up-regulated under salt stress, while PR1a, PR5, PR8 and PR16 were down-regulated.In addition, upstream of these PR genes were analyzed , We find that there are regulatory elements involved in stress response, of which abscisic acid response element (ABRE), TC-rich repeats and HSE appear more frequently.These protein expression data further substantiate the correlation between PR protein and stress response It plays an important and different role.