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应用D1E5单克隆抗体(AB1,为小鼠IgM McAb,能识别马来丝虫感染期幼虫表面抗原决定簇)发现有一期特异性表面抗原仅存在于马来丝虫第二期后期和第三期幼虫。感染动物后2～3天内该抗原即从虫体表面丧失。它可能与丝虫传播有关和/或可以作为免疫攻击的靶抗原,然而分离和鉴定这种抗原的工作均未成功,因此制备含有D1E5独特型“内影像”的兔抗独特型抗体来替代该抗原。将AB1免疫兔产生抗独特型抗体(AB2),继用经典竞争试验检测AB2抗独特型特异性,结果表明AB2能特异性抑制AB1与马来丝虫幼虫表面结合,而不能抑制犬恶
Application of D1E5 monoclonal antibody (AB1, a mouse IgM McAb that recognizes surface antigenic determinants of larvae of the Malayi worm) found that a specific surface antigen was present only in the second and third phases of the malayian filariasis larva. The antigen is lost from the surface of the parasites within 2 to 3 days after infection. It may be related to filarial transmission and / or may serve as a target antigen for immunological challenge, however, neither the isolation nor identification of this antigen has been successful, so a rabbit anti-idiotypic antibody containing a D1E5 idiotype “internal image” antigen. Anti-idiotypic antibody (AB2) was produced in rabbits immunized with AB1 and anti-idiotypic type AB2 was tested by classical competition test. The results showed that AB2 could specifically inhibit the binding of AB1 to the surface of larvae of Malayan filariasis,