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AIM:To investigate the patterns of cell proliferation inproximal and distal colons in normal rats and rats with 1,2-dimethylhydrazine(DMH)induced carcinogenesis using thethymidine analogue bromodeoxyuridine.METHODS:Colonic crypt cell proliferation wasimmunohistochemically detected using the anti-bromodeoxyuridine Bu20a monoclonal antibody.RESULTS:Marked regional differences were found in bothgroups.Total labelling index(LI)and proliferative zone sizein both normal(8.65±0.34 vs 7.2±0.45,27.74±1.07 vs16.75±1.45)and DMH groups(13.13±0.46 vs 11.55±0.45,39.60±1.32 vs35.52±1.58)were significantly higher in distalthan in proximal colon(P<0.05),although the number ofcells per proximal crypt was greater(31.45±0.20 vs 34.45±0.39,42.68±0.53 vs49.09±0.65,P<0.0001).Crypt length,total LI and proliferative zone size all increased in both proximaland distal regions of DMH rats compared to normal controls(P<0.0001).In DMH-treated rat colon a shift of labelled cellsto higher crypt cell positions was demonstrated distally whilsta bi-directional shift was evident proximally(P<0.05).CONCLUSION: Our results show that changes in cell proliferation patterns, as assessed by bromodeoxyuridine uptake, can act as a reliable intermediate marker of colonic cancer formation. Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in the distal colon.
AIM: To investigate the patterns of cell proliferation in mouse and distal colons in normal rats and rats with 1,2-dimethylhydrazine (DMH) induced carcinogenesis using the thymidine analogue bromodeoxyuridine. METHODS: Colonic crypt cell proliferation was immunohistochemically detected using the anti-bromodeoxyuridine Bu20a monoclonal antibody .RESULTS: Marked regional differences were found in both groups. Total LOLING index (LI) and proliferative zone sizein both normal (8.65 ± 0.34 vs 7.2 ± 0.45, 27.74 ± 1.07 vs 16.75 ± 1.45) and DMH groups (13.13 ± 0.46 vs 11.55 ± 0.45, 39.60 ± 1.32 vs35.52 ± 1.58) were significantly higher in distalthan in the proximal colon (P <0.05), although the number of cells per proximal crypt was greater (31.45 ± 0.20 vs 34.45 ± 0.39, 42.68 ± 0.53 vs 49.09 ± 0.65, P <0.0001) .Crypt length, total LI and proliferative zone size all increased in both proximaland distal regions of DMH rats compared to normal controls (P <0.0001) .In DMH-treated rat colon a shift of labeled cellsto higher crypt cell posit ions was demonstrated distally¾¾ bi-directional shift was shown proximally (P <0.05). CONCLUSION: Our results show that changes in cell proliferation patterns, as assessed by bromodeoxyuridine uptake, can act as a reliable intermediate marker of colonic cancer formation. Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in the distal colon.