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目的探讨不同时期高脂日粮小鼠股骨性能变化,及白藜芦醇、槲皮素的干预效果。方法 4 w龄C57BL/6雌性小鼠,随机分成4组:正常日粮组(C)、高脂日粮组(HFD,含20%脂肪)、高脂日粮+0.06%白藜芦醇组(HFD+0.06%R)和0.01%槲皮素组(HFD+0.01%Q)。分别在实验3w、6w、13w、26w末处死小鼠,测定股骨参数、氧化应激指标、荧光定量PCR检测6w、26w相关基因ctsk、BGLAP2、col1a1表达。结果与同期C组相比,HFD组股骨丙二醛6w至26w显著增高,GSH/GSSG在6w、26w显著降低(P<0.05)、13w时略有恢复;HFD组小鼠股骨灰分与GSH/GSSG呈现相似趋势;股骨钙含量自13w开始显著低于C组,持续到26w(P<0.05);股骨长度和骨强度在26w显著低于C组(P<0.05);HFD组股骨成骨相关基因col1a1、BGLAP2显著下调、骨基质降解相关基因ctsk显著上调(P<0.05)。白藜芦醇和槲皮素减轻同期高脂组股骨氧化应激,调节ctsk、BGLAP2、collal表达并改善股骨性能参数。结论在长期高脂膳食饲喂中,小鼠自身股骨发育存在周期性调节;白藜芦醇和槲皮素在各个时期通过改善氧化还原状态保护股骨性能。
Objective To investigate the changes of femur performance and the intervention effects of resveratrol and quercetin in high fat diet mice in different periods. Methods Four-week-old C57BL / 6 female mice were randomly divided into 4 groups: normal diet group (C), high fat diet group (HFD with 20% fat), high fat diet + 0.06% resveratrol group (HFD + 0.06% R) and 0.01% quercetin (HFD + 0.01% Q). Mice were sacrificed at the end of experiment 3w, 6w, 13w and 26w, respectively. Femur parameters and oxidative stress indexes were measured. Expressions of ctsk, BGLAP2 and col1a1 in 6w and 26w were detected by real-time quantitative PCR. Results Compared with group C in the same period, the content of malondialdehyde in femur of HFD group was significantly increased from 6w to 26w, GSH / GSSG was significantly decreased at 6w and 26w (P <0.05), and recovered slightly at 13w. / GSSG showed a similar trend; the femoral calcium content was significantly lower than that of C group from 13w to 26w (P <0.05); the length of femur and bone strength at 26w were significantly lower than that of C group (P <0.05) Related genes col1a1, BGLAP2 were significantly down-regulated, ctsk of bone matrix degradation significantly increased (P <0.05). Resveratrol and quercetin attenuated the femoral oxidative stress in hyperlipidemic rats and regulates the expression of ctsk, BGLAP2, collal and improves the parameters of femur. Conclusion In long-term high-fat diet, there is periodic regulation of mouse femur development. Resveratrol and quercetin protect the femur performance by improving the redox state in different periods.