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目的:探讨COX-2-1195G>A遗传变异与高危型HPV感染交互作用对宫颈癌发病风险的影响。方法:采用PCR-RFLP技术对127例宫颈癌患者和127例健康女性的COX-2-1195位点进行基因分型,以非条件性logistic回归模型计算各基因型与宫颈癌发病关联度,并对宫颈癌患者宫颈刮片HPV16/18检测结果分层分析,以阐释COX-2-1195G>A遗传变异与高危型HPV感染的交互作用。结果:对照组和宫颈癌组COX-2-1195 GG、AG和AA基因型频率分别是:25.2%、53.5%、21.3%和14.2%、55.1%、30.7%,有统计学差异(P=0.047)。宫颈癌组中A等位基因的频率显著高于对照组(58.3%vs48.0%,P=0.021)。回归分析显示,携带一个A等位基因的患病风险为GG型的1.83倍(95%CI:0.94~3.57,P=0.076),携带2个A等位基因的患病风险则为2.57(95%CI:1.20~5.48,P=0.015)倍。宫颈癌患者中,HPV阳性者A等位基因频率较阴性者高,P=0.039。相对于GG基因型、GA基因型OR值为1.50(95%CI:0.53~4.24,P=0.445),AA型基因OR值为4.40(95%CI:1.23~15.72,P=0.023)。结论:COX-2-1195G>A遗传变异与高危型HPV感染协同作用可能是宫颈癌的发病机理之一。
Objective: To investigate the effect of the interaction between COX-2-1195G> A genetic variation and high-risk HPV infection on the risk of cervical cancer. Methods: The genotypes of COX-2-1195 in 127 cases of cervical cancer and 127 healthy women were genotyped by PCR-RFLP. The incidence of cervical cancer was calculated by non-conditional logistic regression model Cervical cancer patients cervical smear HPV16 / 18 test results stratified analysis to explain the COX-2-1195G> A genetic variation and high-risk HPV infection interaction. Results: The frequencies of COX-2-1195 GG, AG and AA genotypes in control group and cervical cancer group were 25.2%, 53.5%, 21.3% and 14.2%, 55.1% and 30.7% respectively, with statistical significance (P = 0.047 ). The frequency of A allele in cervical cancer group was significantly higher than that in control group (58.3% vs48.0%, P = 0.021). Regression analysis showed that the risk of carrying one A allele was 1.83 times that of the GG type (95% CI: 0.94-3.57, P = 0.076), while the risk of carrying two A alleles was 2.57 (95 % CI: 1.20 ~ 5.48, P = 0.015) times. Cervical cancer patients, HPV-positive A allele frequency was higher than the negative, P = 0.039. The OR of GA genotype was 1.50 (95% CI: 0.53-4.24, P = 0.445), and the OR of AA genotype was 4.40 (95% CI: 1.23-15.72, P = 0.023) relative to GG genotype. Conclusion: The synergistic effect between COX-2-1195G> A genetic variation and high-risk HPV infection may be one of the pathogenesis of cervical cancer.