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目的建立超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定豆芽中6-苄基腺嘌呤、异戊烯腺嘌呤、4-氯苯氧乙酸、4-氟苯氧乙酸、吲哚乙酸、吲哚丁酸等6种植物生长调节剂(PGR)残留量的方法。方法豆芽经乙腈及Qu ECh ERS提取包提取,C_(18)粉末净化、离心,色谱柱为WATERS ACQUITY UPLC BEH C_(18)柱(100 mm×2.1 mm,1.7μm),样液经超纯水10倍稀释后,采用三重四级杆质谱的多反应监测模式(MRM)进行测定,基质匹配标准溶液外标法定量。结果 6种PGR在各自浓度范围内线性关系良好(r>0.998)。以空白豆芽为基质进行3个水平加标回收实验,回收率为84.2%~107.5%,相对标准偏差为3.08%~12.71%(n=6),方法检出限为0.03~3.0μg/kg,定量限为0.1~10.0μg/kg。结论该法操作简便、有机试剂使用量少、灵敏度高、稳定性好,适用于豆芽中6种植物生长调节剂残留量的检测要求。
OBJECTIVE To establish a method for the simultaneous determination of 6-benzyladenine, isopentenyladenine, 4-chlorophenoxyacetic acid, 4-fluorophenoxyacetic acid and indole in sprouts by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) Acetic acid, indole butyric acid and other six kinds of plant growth regulators (PGR) residues. Methods The sprouts were extracted by extraction with acetonitrile and Qu ECh ERS. The C 18 powder was purified and centrifuged. The sample was separated by WATERS ACQUITY UPLC BEH C 18 column (100 mm × 2.1 mm, 1.7 μm) After 10-fold dilution, the assay was performed in a multi-reaction monitoring mode (MRM) with triple quadrupole mass spectrometry and external standard method with matrix matching standard solution. Results Six kinds of PGR had a good linear relationship (r> 0.998) in their respective concentration ranges. The recoveries were 84.2% -107.5% and the relative standard deviations were 3.08% ~ 12.71% (n = 6). The detection limits were 0.03-3.0 μg / kg, Quantitative limit of 0.1 ~ 10.0μg / kg. Conclusion The method is simple, less use of organic reagents, high sensitivity, good stability, suitable for the detection of 6 kinds of plant growth regulators in bean sprouts.