Chronic neuroprotective effects of low concentration lithium on SH-SY5Y cells:possible involvement o

• 作 者: Riadh Nciri Ezzeddine Bourogaa Samira Jbahi Mohamed Salah Allagui Abdelfattah Elfeki Christian Vincent Franoise Croute
• 机 构: Cellular Biology Laboratory, Purpan Medicine Faculty, Paul Sabatier University, Toulouse, France“,”E
• 出 处: Neural Regeneration Research
• 发表日期: 2014年7期
• 关键词: lithium translational calmodulin hydroxylase pyruvate carbonate dopamine likely

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate (Li2CO2) for 25-50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2 (PKM2) and calmodulin 3 (CaM 3) expression levels were signiifcantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II (CK2), threonine/tyrosine phosphatase 7 (PYST2), and dopamine beta-hydroxylase (DBH) expression levels were signiifcantly up-regulated. Besides, west blot analysis of stress proteins (HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins:a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modiifcations of proteins cited above.