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本文介绍了两种微量血痕的 MN 血型检验方法。1.低温减压解离法的要点是:(1)剪取0.3～0.5cm 长的血痕纱线,用甲醇固定并分离;(2)置凹玻璃板凹内,加适合于检验血痕血型的抗 M、抗 N 血清;(3)在负压760mmHg 环境中吸收20分钟,再置4℃环境中吸收20～30分钟;(4)将凹玻璃板置冰块上用冷盐水洗涤血痕纱线;(5)吸干洗涤过的血痕纱线,滴加0.5～1%相应型的红细胞盐水悬液,减压解离20分钟;(6)用镊子夹起血痕纱线放在有两滴盐水的载玻片上,加盖玻片;(7)室温20分钟后,用显微镜观察。2.载片热解离法基本同第一法。不同点如下:(1)经洗涤后的纱线置载玻片上,加相应型的0.05%盐水红细胞悬液;(2)在56℃环境中解离10分钟。本文还测定了低温减压解离法的灵敏度,并对其室温解离原理进行了讨论。
This article describes the MN blood type test method of two trace bloodstains. 1. Low-temperature decompression dissociation of the main points are: (1) cut 0.3 ~ 0.5cm long bloodstained yarn, fixed and separated with methanol; (2) concave glass plate concave, plus suitable for testing blood group blood type Anti-M, anti-N serum; (3) absorb negative pressure 760mmHg environment for 20 minutes and then placed in 4 ℃ environment for 20 to 30 minutes; (4) the concave glass plate was placed on ice with cold saline washing bloodstain ; (5) suck dry washed bloodstain yarn, 0.5-1% corresponding drops of erythrocyte saline solution, decompression dissociation 20 minutes; (6) pick up the bloodstains with tweezers on the yarn with two drops of saline Slide, cover glass slide; (7) after 20 minutes at room temperature, with a microscope. 2. Carrier thermal dissociation method with the basic law. The differences are as follows: (1) After washing the yarn placed on the slide, add the corresponding 0.05% saline erythrocyte suspension; (2) dissociation in 56 ℃ environment for 10 minutes. The sensitivity of cryogenic vacuum dissociation method was also measured, and the principle of room temperature dissociation was also discussed.