论文部分内容阅读
目的:观察2型糖尿病(T2DM)大鼠肾损伤过程中血管紧张素Ⅱ(AngⅡ)的表达,探讨通心络对糖尿病早期肾小球高滤过的影响及其作用机制。方法:8周龄SD雄性大鼠40只,采用高脂饲料+低剂量链脲佐菌素建立T2DM大鼠模型,随机分为空白组、模型组、缬沙坦组(10 mg·kg-1·d-1)和通心络组(0.4 mg·kg-1·d-1),分别于4周和8周观察T2DM大鼠血糖(FBG)、尿白蛋白排泄率(UAER)及肾功能(Scr,BUN,Ccr)的变化;8周末次给药后取肾组织,计算肾重指数(KW/BW),采用western blot及real time-PCR技术检测AngⅡ和AngⅡ1型受体(AT1R)蛋白和基因的表达。结果:T2DM大鼠的FBG、UAER、Scr、BUN、Ccr及KW/BW较空白组有不同程度的升高(P<0.05),通心络组FBG无明显变化(P>0.05),其他各指标水平较模型组均显著降低(P<0.05);WB及RT-PCR结果显示通心络可以降低肾组织中AngⅡ和AT1R基因蛋白的过度表达(P<0.05)。结论:通心络可以改善T2DM大鼠早期的高滤过状态,抑制尿白蛋白的排泄及肾脏肥大,保护肾功能;通心络可能是通过下调肾组织中AngⅡ的表达,抑制异常活化的RAS来延缓糖尿病肾病的发生发展。
Objective: To observe the expression of angiotensin Ⅱ (AngⅡ) in the process of renal injury in type 2 diabetes mellitus (T2DM) rats and to explore the effect of Tongxinluo on glomerular hyperfiltration in early stage of diabetes mellitus and its mechanism. Methods: Forty male Sprague-Dawley rats of 8 weeks old were randomly divided into blank group, model group, valsartan group (10 mg · kg -1) and low-dose streptozotocin D-1) and Tongxinluo (0.4 mg · kg-1 · d-1) respectively. The blood glucose (FBG), urinary albumin excretion (UAER) and renal function (Scr, BUN and Ccr). At the end of the 8th week after administration, the kidneys were taken out to calculate the kidney weight index (KW / BW). Western blot and real time-PCR were used to detect the expression of AngⅡ and AngⅡ1 receptor (AT1R) And gene expression. Results: FBG, UAER, Scr, BUN, Ccr and KW / BW in T2DM rats were significantly higher than those in blank control group (P <0.05) (P <0.05). The results of WB and RT-PCR showed that Tongxinluo can reduce the over-expression of AngⅡ and AT1R protein in renal tissue (P <0.05). Conclusion: Tongxinluo can improve early hyperfiltration state of T2DM rats, inhibit urinary albumin excretion and renal hypertrophy, and protect renal function; Tongxinluo may be through down-regulation of Ang Ⅱ expression in renal tissue, inhibition of abnormal activation of RAS To delay the occurrence and development of diabetic nephropathy.