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目的建立酒类中安赛蜜、糖精钠、甜蜜素和三氯蔗糖4种甜味剂的超高压液相色谱-串联质谱联用(UPLC-MS/MS)快速测定方法。方法采用AgilentZORBAXSB-C18色谱柱(50mm×2.1mm,1.8μm)分离,以0.1%甲酸水溶液-甲醇作为流动相梯度洗脱,在电喷雾离子源ESI负离子模式下多重反应监测(MRM)定性、定量测定甜味剂。结果 4种甜味剂在10～500μg/L的范围内与峰面积有良好的线性关系,相关系数大于0.99,检出限为0.5～2.4μg/L,加标回收率在89.4%～104.6%之间,RSD小于4%。结论该方法简便灵敏,准确快速,可用于酒类食品中低剂量、复合甜味剂的测定。
OBJECTIVE To establish a method for the rapid determination of four kinds of sweeteners including acesulfame potassium, saccharin sodium, cyclamate and sucralose by UPLC-MS / MS. Methods The separation was carried out on an Agilent ZORBAXSB-C18 column (50 mm × 2.1 mm, 1.8 μm). The mobile phase was eluted with 0.1% formic acid in methanol as mobile phase. Multiple reaction monitoring (MRM) Determination of sweeteners. Results Four kinds of sweeteners had a good linear relationship with the peak area in the range of 10 ~ 500 μg / L, the correlation coefficient was greater than 0.99, the detection limit was 0.5 ~ 2.4 μg / L and the recoveries were 89.4% -104.6% Between, RSD is less than 4%. Conclusion The method is simple, sensitive, accurate and rapid and can be used for the determination of low dose and complex sweeteners in alcoholic foods.