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目的:探讨父源性HLA-G基因14bp缺失多态性与早发型重度子痫前期的相关性。方法:采用PCR方法,对中国地区汉族人群中40对早发型重度子痫前期父儿和50对正常晚孕者父儿进行HLA-G基因第8外显子14bp缺失多态性的等位基因分型,比较两组父亲之间和两组新生儿之间等位基因及基因型的频率分布,通过父/儿基因型配伍,比较两组间基因型配伍频率分布的差异。结果:①早发型重度子痫前期组新生儿HLA-G-14bp频率52.5%和-14bp/-14bp基因型频率显著低于对照组(P=0.024;P=0.010);②早发型重度子痫前期组父(-14bp/-14bp)/儿-14bp/-14bp基因型配伍频率显著低于对照组(P=0.013);③早发型重度子痫前期父亲组HLA-G 14bp缺失多态性的等位基因频率分布与正常对照组比较差异虽无统计学意义,但是有差异性趋势(P=0.051)。结论:父源性HLA-G基因14bp缺失多态性可能与早发型重度子痫前期的发病相关,父(-14bp/-14bp)/儿-14bp/-14bp基因型配伍可能会降低母亲患重度子痫前期的风险。
Objective: To investigate the correlation between the 14 bp deletion polymorphism of paternal HLA-G gene and early-onset severe preeclampsia. Methods: The alleles of the 14 bp deletion polymorphism of exon 8 of HLA-G gene in 40 pairs of early-onset severe preeclampsia and 50 pairs of normal pregnant women in Chinese Han population were analyzed by PCR The frequency distribution of alleles and genotypes between two groups of fathers and between two groups of newborns was compared. The frequency distribution of genotype compatibility between the two groups was compared by parent-child genotype compatibility. Results: (1) The frequencies of HLA-G-14bp in neonates with early-onset severe preeclampsia were 52.5% and -14bp / -14bp, respectively, significantly lower than those in control group (P = 0.024; P = 0.010) The compatibility frequency of the -14bp / -14bp / -14bp / -14bp genotypes in the pre-term group was significantly lower than that in the control group (P = 0.013); ③The HLA-G 14bp deletion polymorphism Allele frequency distribution of the control group, although the difference was not statistically significant, but there is a trend of difference (P = 0.051). CONCLUSION: The 14 bp deletion polymorphism of paternal HLA-G gene may be associated with the onset of severe preeclampsia. The compatibility of the parent (-14bp / -14bp) / children with -14bp / -14bp genotype may reduce the severity of maternal illness Risk of preeclampsia.