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目的利用DW I及PW I动态观察缺血前预注川芎嗪(TMP)对大鼠缺血后脑实质的系列变化,探讨功能磁共振成像在脑缺血神经保护药物的作用机理、疗效监测方面的应用价值。方法建立大鼠急性脑缺血再灌注模型,30只雄性SD大鼠(280~320 g),随机分成2组(每组,n=15):实验组(A组)和对照组(B组),分别于缺血前30 m in腹腔注射TMP 100 mg/kg和生理盐水1 m l。A组、B组分别于阻闭2 h后抽出尼龙线,恢复再灌注。于再通前,再通后1、2、3、6、12 h及24 h行功能及常规磁共振扫描。对2组结果进行比较分析。结果(1)DW I显示在缺血前腹腔注射TMP 100 mg/kg可明显缩小缺血后各时间点脑缺血面积,与对照组相比,差异有统计学意义(Ρ<0.05)。(2)急性期病灶中心血流灌注减少程度2组间差异无统计学意义(P>0.05)。结论DW I及PW I可以很好地观察TMP对大鼠缺血后脑实质保护作用的动态变化,显示功能磁共振在脑缺血神经保护药物的作用机理、疗效监测等方面具有极大的应用潜力。
OBJECTIVE: To dynamically observe the serial changes of brain parenchyma after ischemic preconditioning with preconditioning of TMP in rats by DWI and PWI, and to explore the mechanism of functional magnetic resonance imaging in neuroprotective agents of cerebral ischemia and the monitoring of therapeutic effect Value. Methods Acute cerebral ischemia-reperfusion model in rats was established. Thirty male SD rats (280-320 g) were randomly divided into 2 groups (n = 15 in each group): experimental group (A) and control group ) Were injected intraperitoneally with TMP 100 mg / kg and saline 1 ml respectively 30 min before ischemia. A group, B group were blocked after blocking 2 h nylon line, reperfusion. Before re-pass, and after 1, 2, 3, 6, 12 h and 24 h line function and conventional magnetic resonance scan. Two groups of results for comparative analysis. Results (1) DWI showed that ischemic preconditioning of TMP 100 mg / kg significantly reduced the area of cerebral ischemia at each time point after ischemia. Compared with the control group, the difference was statistically significant (P <0.05). (2) There was no significant difference between the two groups in the reduction of central perfusion in acute focal lesion (P> 0.05). Conclusions DW I and PW I can well observe the dynamic changes of TMP on ischemic brain parenchyma in rats, showing that FMR has great potential in the mechanism of neuroprotective effect of cerebral ischemia and monitoring of therapeutic effect .