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目的:检测微卫星不稳定与上皮性卵巢癌的关系。方法:对卵巢上皮性癌患者的临床资料做回顾性分析,分析卵巢癌患者的发病年龄、临床表现、病理分期、组织学分级情况,并利用PCR-SSCP的方法检测卵巢上皮性癌发生微卫星不稳定的频率及与卵巢上皮性癌的病理分期、组织学分级的关系。结果:71例卵巢上皮性癌患者发生微卫星不稳定的频率为64.8%,卵巢良性肿瘤患者和正常对照组频率为0。早期(Ⅰ～Ⅱ)患者和晚期(Ⅲ～Ⅳ)患者中微卫星不稳定发生频率分别为42.3%、77.8%,差异有统计学意义(χ2=10.91,P<0.05);卵巢上皮性恶性肿瘤中G1～G2期、G3期发生MSI的频率分别为50.0%、83.7%,差异有统计学意义(χ2=10.33,P<0.05)。结论:D7S522位点的微卫星不稳定只发生在卵巢癌中,而不发生在卵巢良性肿瘤患者及正常人中;D7S522位点的微卫星不稳定与卵巢上皮性癌的病理分期、组织学分级均有关,D7S522位点的微卫星不稳定参与卵巢上皮性癌的进展,病变越重,D7S522位点的微卫星不稳定发生率越高。
Objective: To detect the relationship between microsatellite instability and epithelial ovarian cancer. Methods: The clinical data of patients with epithelial ovarian cancer were retrospectively analyzed. The age of onset, clinical manifestations, pathological staging and histological grade of patients with ovarian cancer were analyzed. PCR-SSCP was used to detect the occurrence of ovarian epithelial carcinomas The frequency of instability and its relationship with the pathological staging and histological grade of epithelial ovarian cancer. Results: The frequency of microsatellite instability was 64.8% in 71 patients with epithelial ovarian cancer and 0 in patients with benign ovarian tumors and normal controls. The frequency of microsatellite instability was 42.3% and 77.8% in patients with early stage (Ⅰ ~ Ⅱ) and in patients with advanced stage (Ⅲ ~ Ⅳ) respectively (χ2 = 10.91, P <0.05); ovarian epithelial malignancies The frequency of MSI in G1 ~ G2 phase and G3 phase were 50.0% and 83.7%, respectively, with significant difference (χ2 = 10.33, P <0.05). Conclusion: The microsatellite instability of D7S522 locus only occurs in ovarian cancer, but not in patients with benign ovarian tumors and in normal subjects. Microsatellite instability at D7S522 locus and histological grade of ovarian epithelial carcinoma Were related to D7S522 site of microsatellite instability involved in the progress of epithelial ovarian cancer, lesions heavier, D7S522 site, the higher the incidence of microsatellite instability.