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目的研究移植肾急性排斥反应前后外周血淋巴细胞Notch1的表达及意义。方法40例肾移植受者按术后是否出现急性排斥反应分为排斥组(n=20)及无排斥组(n=20)。应用流式细胞技术检测两组术前、术后排斥反应发生时及抗排斥治疗后的外周血淋巴细胞Notch1的表达,观测各组的Notch1阳性淋巴细胞率,对各组各时间点间的测得值进行比较分析。结果排斥组在术后排斥发生时的Notch1阳性淋巴细胞率明显高于术前(t=4.245,P=0.000),也明显高于无排斥组(t=3.839,P=0.000),抗排斥治疗后明显下降(t=3.102,P=0.004);而无排斥组术后的Notch1阳性淋巴细胞率与术前无显著性差异(P=0.409);不同的免疫抑制方案间亦无显著性差异(P>0.05)。结论监测外周血淋巴细胞Notch1的表达有助于诊断急性排斥反应,并可提示抗排斥治疗的效果。
Objective To study the expression and significance of Notch1 in peripheral blood lymphocytes before and after acute rejection of renal allograft. Methods Forty renal transplant recipients were divided into rejection group (n = 20) and non-rejection group (n = 20) according to whether acute rejection occurred after operation. Flow cytometry was used to detect the expression of Notch1 in peripheral blood lymphocytes of both groups before and after rejection and anti-rejection treatment. The rates of Notch1-positive lymphocytes in each group were measured. The value of comparative analysis. Results Exclusion group had significantly higher Notch1 positive lymphocyte rate than that before operation (t = 4.245, P = 0.000), and significantly higher than those without rejection (t = 3.839, P = 0.000) (T = 3.102, P = 0.004). There was no significant difference in the number of Notch1-positive lymphocytes in non-rejection group after surgery (P = 0.409). There was no significant difference between different immunosuppressive regimens P> 0.05). Conclusion Monitoring the expression of Notch1 in peripheral blood lymphocytes may help to diagnose acute rejection and may indicate the effect of anti-rejection therapy.