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目的了解重庆市永川区乡镇3~6岁留守儿童维生素D营养状况,探讨维生素D受体基因FOKⅠ位点多态性与25-羟维生素D[25-(OH)D]的相关性。方法 2015年11月-2016年4月期间,由重庆医科大学附属永川医院招募永川区部分乡镇3~6岁留守儿童进行健康体检,以非留守儿童作为对照,采用液相色谱串联质谱法进行血清25-(OH)D水平检测,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)法进行FOKⅠ位点基因型检测。结果 1285例3~6岁留守儿童血清25-(OH)D平均水平为(19.566±5.185)ng/m L,维生素D充足占2.81%;不足组占41.40%;缺乏占52.98%;严重缺乏占2.81%。留守儿童组血清25-(OH)D较之非留守儿童组低,但两组比较无显著差异。其中3岁组25-(OH)D平均水平最高,各年龄组维生素D水平比较差异有统计学意义(P<0.001)。2ff基因型的维生素D水平较其余2种基因型低,但3种基因型的维生素D水平差异无显著统计学意义(P>0.05)。采用协方差分析校正年龄对维生素D水平的影响后,各基因型维生素D水平相比较差异仍无显著统计学意义(P>0.05)。结论重庆市永川区留守儿童25-(OH)D水平严重低下,需采取措施提高维生素D水平,VDR受体基因FOKⅠ位点多态性可能与25-(OH)D水平无关。
Objective To investigate the nutritional status of vitamin D in left-behind children aged 3 ~ 6 years in Yongchuan District of Chongqing Municipality and to explore the relationship between the polymorphism of FOKⅠ locus in vitamin D receptor gene and 25-hydroxyvitamin D [25- (OH) D]. Methods From November 2015 to April 2016, Yongchuan Hospital Affiliated to Chongqing Medical University recruited left-behind children aged 3 ~ 6 years old in Yongchuan district for physical examination. The non-left-behind children were used as control. Serum was determined by liquid chromatography tandem mass spectrometry 25- (OH) D levels were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method for FOK Ⅰ locus genotype detection. Results The average level of 25- (OH) D in 1285 left-behind children aged 3 to 6 years was (19.566 ± 5.185) ng / m L, vitamin D was 2.81%, the deficiency was 41.40%, the deficiency was 52.98% 2.81%. Left-behind children with serum 25- (OH) D than non-left-behind children group, but no significant difference between the two groups. Among them, the average level of 25- (OH) D in the 3-year-old group was the highest, and there was significant difference in the vitamin D level in all age groups (P <0.001). 2ff genotype had lower vitamin D level than the other two genotypes, but there was no significant difference in vitamin D levels between the three genotypes (P> 0.05). After covariance analysis was used to correct the effect of age on vitamin D levels, there was no significant difference in the level of vitamin D between different genotypes (P> 0.05). Conclusion The level of 25- (OH) D in children left behind in Yongchuan District of Chongqing Municipality is seriously low. Measures to increase the vitamin D level are needed. The FOKⅠ polymorphism of VDR receptor gene may not be related to the level of 25- (OH) D.