中药治疗大鼠胃缺血再灌粘膜损伤

• 作 者: 赵子厚 马丽红 张锁雅 梁代英 马晓立 李春水
• 机 构: 北京市中医研究所药理室
• 出 处: 新消化病学杂志
• 发表日期: 1997年09期
• 关键词: 胃粘膜损伤 苍脂颗粒剂 大鼠 胃疾病 脂质过氧化 中医药疗法 氧自由基 细胞保护作用 再灌注损伤 病理模型组

目的探讨实验性缺血后再灌注大鼠胃粘膜损伤与氧自由基脂质过氧化的关系及中药苍脂颗粒剂对胃粘膜损伤的细胞保护作用机制．方法大鼠腹腔动脉结扎，造成胃缺血２０ｍｉｎ后解除结扎，再灌注６０ｍｉｎ，取出胃粘膜，观察对照组、病理对照组及中药各组诸项指标变化．结果大鼠缺血再灌注后，胃粘膜ＬＰＯ含量比对照组明显增加，ＳＯＤ（１５９３±３２１→８２７±１７０ＮＵ／ｍｇ蛋白），ＮＰＳＨ（０４１±００９→０２２±００５ｍｍｏｌ／ｍｇ蛋白），ＧＳＨＰＸ（２６９±３１→１８７±２２Ｕ／ｍｇ蛋白）含量降低（Ｐ＜００１）；术前口服苍脂颗粒剂组动物的胃粘膜ＬＰＯ含量（１２１±６１ＦＵ／ｍｇ蛋白）明显低于病理组（１９７±６０ＦＵ／ｍｇ蛋白），ＳＯＤ（８２７±１７０→１４６５±４７４ＮＵ／ｍｇ蛋白），ＮＰＳＨ（０２２±００５→０４３±００８ｍｍｏｌ／ｍｇ蛋白），ＧＳＨＰＸ（１８７±２２→２４２±５７Ｕ／ｍｇ蛋白）含量亦显著增加（Ｐ＜００５，Ｐ＜００１），而ＣＡＴ和ＰＧＥ２含量在实验各组的比较无统计学意义（Ｐ＞００５）．结论氧自由基脂质过氧化参与胃缺血再灌注后的胃粘膜损伤过程，苍?

Objective To investigate the relationship between gastric mucosal lesion and oxygen free radical lipid peroxidation in experimental ischemia reperfusion rats and the protective mechanism of CGT on gastric mucosal injury. Methods The ligation of celiac artery in rats resulted in the ligation of gastric ischemia for 20min, the reperfusion for 60min and the gastric mucosa removed. The indexes of the control group, pathological control group and TCM groups were observed. Results The content of LPO in gastric mucosa increased significantly after ischemia-reperfusion in rats, and SOD (1593 ± 321 → 827 ± 170NU / mg protein) and NPSH (041 ± 009) → 022 ± 005 mmol / mg protein), GSHPX (269 ± 31 → 187 ± 22 U / mg protein) decreased (P <001) The content of LPO (121 ± 61FU / mg protein) in gastric mucosa was significantly lower than that in pathological group (197 ± 60FU / mg protein) and SOD (827 ± 170 → 1465 ± 474 NU / mg protein), NPSH (022 ± 005 → 043 ± 008 mmol / mg protein), GSHPX (187 ± 22 → 242 ± 5  7U / mg protein) also significantly increased (P <0  05, P <0  01), while CAT and PGE2 content in the real There was no significant difference between the groups (P> 005). Conclusion Oxygen-free radical lipid peroxidation participates in gastric mucosal lesion process after gastric ischemia-reperfusion.