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利用根癌农杆菌 (Agrobacteriumtumefaciens)介导 ,首次将菜豆几丁质酶和烟草β 1 ,3 葡聚糖酶双价水解酶基因导入番茄品种A5 3(Lycopersiconescu lentumcv .A5 3)中 ,获得批量转基因再生植株。对外源基因的PCR和Southern杂交结果表明 ,外源基因已经整合到番茄基因组中 ,其拷贝数 1～ 8个不等。Northern杂交和卡那霉素喷施表明目的基因和标记基因均已得到表达 ,抗病性鉴定初步表明转基因植株对枯萎病的抗性显著提高。
Using Agrobacterium tumefaciens-mediated transformation, the genes of bean chitinase and tobacco β 1, 3 glucanase bivalent hydrolase were introduced into Lycopersicon esculentum A5 (Lycopersiconescu lentumcv. Regenerate plants. The results of PCR and Southern hybridization of the exogenous genes showed that the exogenous gene has been integrated into the genome of tomato and the copy number ranged from 1 to 8. Northern blotting and kanamycin spraying indicated that both the target gene and the marker gene had been expressed. The identification of disease resistance showed that the resistance of transgenic plants to Fusarium wilt was significantly increased.