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为筛选茄子(Solanum melongena L.)高温胁迫下稳定表达的内参基因,以不同茄子品系(种)为研究对象,利用实时荧光定量PCR技术对来自茄子高温胁迫转录组数据库8个候选内参基因(SmEF1a、SmEF2、Sm40s RPS29、Sm60s RPL24、SmTRX、SmCK I、SmDAHPS I、SmUCP)和SmActin在不同试验情况下进行表达检测,并结合GeNorm、Norm Finder、Best Keeper和ReFinder软件综合评价9个内参基因的表达稳定性。结果表明,在茄子热敏品系05-1和耐热品系05-4经高温处理不同时间的样品和不同组织样品以及10个耐热性不同的茄子品系(种)中,9个内参基因的表达丰度及稳定性存在差异;茄子热敏品系05-1和耐热品系05-4高温胁迫处理不同时间样品中表达稳定性最好的是SmEF1a和SmUCP;其不同组织中表达水平最稳定的是SmEF1a和SmTRX;高温胁迫下不同茄子品系(种)中以SmTRX和SmEF2的表达稳定性最好。综合来看,SmEF1a和SmTRX在所有茄子试验样品中的表达稳定性最好,而SmActin和SmCK I的表达稳定性较差。
In order to screen the endogenous reference genes stably expressed under high temperature stress in eggplant (Solanum melongena L.), the different eggplant strains (strains) were selected as research objects. Eight candidate reference genes (SmEF1a , SmEF2, Sm40s RPS29, Sm60s RPL24, SmTRX, SmCK I, SmDAHPS I, SmUCP) and SmActin were detected under different experimental conditions, and the expression of nine internal control genes was evaluated with GeNorm, Norm Finder, Best Keeper and ReFinder software stability. The results showed that in the heat-sensitive lines 05-1 and 05-4 of eggplant treated with high temperature for different time samples and different tissue samples and 10 heat-resistant eggplant strains (lines), the expression of 9 internal reference genes Abundance and stability of eggplant; heat-sensitive strain of eggplant 05-1 and heat-resistant strain of 05-4 heat-treated at different times, the expression stability of SmEF1a and SmUCP is the best; the most stable expression level in different tissues is SmEF1a and SmTRX. The expression stability of SmTRX and SmEF2 was the best in different eggplant lines under high temperature stress. Taken together, the expression stability of SmEF1a and SmTRX was the best in all eggplant test samples, while the expression stability of SmActin and SmCK I was poor.