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目的探讨癌基因K-ras及C-myc在宫颈癌中的表达及临床意义。方法选择2010年12月-2016年6月在该院因TCT结果异常或HPV-DNA检测异常行阴道镜宫颈活检术的389例患者作为研究对象,宫颈慢性炎症105例,宫颈上皮内瘤变210例,宫颈癌74例。采用免疫组化方法检测宫颈组织中K-ras及C-myc蛋白的表达水平。结果宫颈癌C-myc、K-ras阳性表达率最高,其次为CINⅢ组,慢性炎症无阳性表达,组间比较差异有统计学意义(P<0.05)。低分化、有淋巴结转移、临床分期为Ⅲ~Ⅳ期的宫颈癌组织中C-myc、K-ras阳性率显著高于高分化、无淋巴结转移以及临床分期为Ⅰ~Ⅱ期的宫颈癌组织,差异有统计学意义(P<0.05)。相关性分析显示,C-myc蛋白与K-ras蛋白表达呈显著正相关的关系(r=0.683,P=0.000)。结论癌基因C-myc以及K-ras参与了宫颈癌的发生及发展过程,并且恶性程度越高,临床分期越晚,其阳性表达率越高。
Objective To investigate the expression and clinical significance of oncogenes K-ras and C-myc in cervical cancer. Methods 389 patients with colposcopic cervical biopsy due to abnormal TCT results or HPV-DNA abnormalities in this hospital from December 2010 to June 2016 were enrolled in this study. 105 cases of chronic cervical inflammation and 210 cases of cervical intraepithelial neoplasia For example, 74 cases of cervical cancer. The expression of K-ras and C-myc in cervical tissue was detected by immunohistochemistry. Results The positive rates of C-myc and K-ras in cervical cancer were the highest, followed by those in CINⅢ group. There was no significant difference between the two groups (P <0.05). The positive rates of C-myc and K-ras in cervical cancer tissues with poorly differentiated lymph node metastasis and stage Ⅲ-Ⅳ were significantly higher than those in well-differentiated, non-lymph node metastatic and stage Ⅰ-Ⅱ cervical cancer tissues, The difference was statistically significant (P <0.05). Correlation analysis showed that there was a significant positive correlation between C-myc protein and K-ras protein expression (r = 0.683, P = 0.000). Conclusion The oncogenes C-myc and K-ras are involved in the carcinogenesis and progression of cervical cancer. The higher the malignancy and the later the clinical stage, the higher the positive rate.