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目的:探讨一氧化氮合酶(NOS)阳性神经元与先天性巨结肠发生的关系。方法:对扩张段和移行段分别在双目解剖显微镜下做全层铺片,以NADPH-d酶组化法着色,光学显微镜下观察并以医学图像测算软件对神经元的面积、光密度等作量化处理。结果:扩张段神经节和神经纤维排列层次明显,色深量多,节内神经元色深,沿神经节周边及突起的基部排列。移行段神经节及神经纤维杂乱,量少色浅,节内神经元多浅染或未染,着色程度有较大差异。移行段近端、中段、远端神经元着色依次减弱。结论:NOS阳性神经元分布与代谢异常使肠壁舒张不能,部分导致了先天性巨结肠的发生。
Objective: To investigate the relationship between nitric oxide synthase (NOS) positive neurons and Hirschsprung’s disease. Methods: The full-thickness lamina was made under the binocular dissection microscope in the dilatation segment and the transitional segment, and then stained with NADPH-d enzyme. The area and optical density of the neurons were observed by optical microscope For quantitative processing. Results: The arrangement of ganglion and nerve fiber in the dilatation segment was obvious. The color depth and depth were deep, and the neurons were darker in color along the periphery of the ganglion and the base of the protrusion. Migration of ganglion and nerve fibers messy, the amount of less light, more or less sections of the neurons with or without dyeing, coloring a greater degree of difference. The migration of proximal, middle, distal neuronal coloring in turn weakened. Conclusion: The distribution and metabolism of NOS positive neurons can not make the intestine wall diastolic, which partly leads to the occurrence of Hirschsprung ’s disease.