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目的:观察雷帕霉素对肝癌细胞生长、凋亡及DDAH2蛋白表达的影响。方法:人肝癌HepG2细胞用不同浓度雷帕霉素(0,4,20,100 nmol/L)分别作用48 h后,用流式细胞法检测细胞周期和凋亡率,用Western blot法检测DDAH2蛋白的表达。结果:与对照组(雷帕霉素0 nmol/L)HepG2细胞比较,各浓度雷帕霉素(4,20,100 nmol/L)处理组HepG2细胞出现明显的G1期阻滞,凋亡率增加,及DDAH2蛋白表达降低(均P<0.05),且均呈一定的浓度依赖性。结论:雷帕霉素能抑制肝癌细胞增殖并促进细胞凋亡,其机制可能与下调DDAH2表达有关。
Objective: To observe the effect of rapamycin on hepatocellular carcinoma cell growth, apoptosis and DDAH2 protein expression. Methods: HepG2 cells were treated with different concentrations of rapamycin (0, 4, 20, 100 nmol / L) for 48 h respectively. Cell cycle and apoptosis rate were detected by flow cytometry. Western blot was used to detect DDAH2 protein expression. Results: Compared with HepG2 cells treated with rapamycin (0 nmol / L), G1 phase arrest and apoptosis were observed in HepG2 cells treated with different concentrations of rapamycin (4,20,100 nmol / L) And DDAH2 protein expression decreased (all P <0.05), and all showed a certain concentration-dependent manner. Conclusion: Rapamycin can inhibit the proliferation of hepatocellular carcinoma cells and promote apoptosis. The mechanism may be related to the down-regulation of DDAH2 expression.