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为了制备抗重组人孕激素受体单克隆抗体，用聚合酶链反应（ＰＣＲ）扩增人孕激素受体氨基端编码区段，定位克隆连入高效表达载体ＰＭＳ－３１ｂ，构建重组质粒ＰＭＳ－ＰＲａ，将其转入大肠杆菌ＰＯＰ２１３６，经３０℃扩增和４２℃温度诱导，表达出分子量为３３ｋＤ的ＭＳ２－ＰＲａ融合蛋白。摇菌液的蛋白表达量约为３０ｍｇ／Ｌ。用这种蛋白免疫ＢＡＬＢ／ｃ小鼠，制备出４株抗人孕激素受体的单克隆抗体。它们用于乳腺癌石蜡和冰冻切片的免疫组织化学染色，较国外制备的单克隆抗体质量更好
In order to prepare anti-recombinant human progestin receptor monoclonal antibody, the coding region of human progesterone receptor was amplified by polymerase chain reaction (PCR), and inserted into the high expression vector PMS-31b to construct the recombinant plasmid PMS- PRa was transformed into E.coli POP2136. After induced by temperature 30 ℃ and temperature 42 ℃, MS2-PRa fusion protein with a molecular weight of 33 kD was expressed. Vibrio fungus protein expression of about 30mg / L. BALB / c mice were immunized with this protein to prepare 4 monoclonal antibodies against human progesterone receptor. They are used for immunohistochemical staining of paraffin and frozen sections of breast cancer and are of better quality than monoclonal antibodies prepared abroad