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为了明确非酶抗氧化物质抗坏血酸(AsA)、还原型谷胱甘肽(GSH)及相关代谢酶抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)在紫花苜蓿(Medicago sativa L.)对牛角花齿蓟马Odontothrips loti Haliday为害的抗性中的作用,测定了不同牛角花齿蓟马虫口密度下抗、感蓟马苜蓿无性系R-1、I-1的AsA、GSH含量及APX、GR活性的变化。结果表明:受牛角花齿蓟马为害后,R-1无性系在低虫口密度(1、3头/枝条)下,AsA、GSH含量和GR活性均上升,在高虫口密度(5、7头/枝条)下,AsA含量和GR活性先升高后下降,GSH含量上升后保持稳定;I-1无性系的AsA、GSH含量先升高后下降,GR活性在为害后期呈上升趋势;R-1、I-1无性系的APX活性均先上升后下降,但R-1无性系APX活性的上升速率及下降速率小于I-1无性系。说明AsA、GSH含量及APX、GR活性的升高可能是紫花苜蓿对牛角花齿蓟马诱导抗性的一种表现,但I-1无性系对蓟马为害的应激反应滞后于R-1无性系。在牛角花齿蓟马为害后期,R-1无性系体内的AsA、GSH含量及APX、GR活性仍处于较高水平,也说明了R-1无性系对牛角花齿蓟马为害的抗性较I-1无性系强。
In order to clarify the effects of non-enzymatic antioxidants AsA, GSH and APX, glutathione reductase (GR) in alfalfa (Medicago sativa L ) On the resistance of Odontothrips loti Haliday to the Asl and GSH contents in the resistant and susceptible alfalfa clones R-1 and I-1 at different insect populations And APX, GR activity changes. The results showed that the content of AsA, GSH and the activity of GR in R-1 clones increased at low insect population density (1, 3 heads / shoots) / Shoots), AsA content and GR activity firstly increased and then decreased, while GSH content increased steadily. AsA and GSH contents in I-1 clones increased first and then decreased, and GR activity increased at the later stage of damage; R- 1, the APX activities of I-1 clones first increased and then decreased, but the R-1 clones APX activity increased and declined at a slower rate than the I-1 clones. These results suggest that the increase of AsA, GSH content and APX, GR activity may be a manifestation of induction resistance of Medicago falcata to H. tabaci, however, the stress response of the I-1 clones to T. thrips lags behind that of R-1 Clones. At the late stage of damage, the content of AsA and GSH and the activities of APX and GR in R-1 clones were still high, which also indicated that R-1 clones were more resistant to H. tabaci. I-1 clones strong.