不同商品规格温厚中厚朴酚与和厚朴酚的含量测定

• 作 者: 冯劼 吴晓宁 余陈欢 方杰
• 机 构: 杭州市红十字会医院,浙江医学高等专科学校,浙江中医药大学,
• 出 处: 中华中医药学刊
• 发表日期: 2011年06期
• 关键词: 温朴 厚朴酚 HPLC法 含量测定 商品规格 厚朴 厚朴酚类 凹叶厚朴 厚朴药材 线性关系

目的:建立不同商品规格温朴中厚朴酚与和厚朴酚含量的高效液相测定方法,测定不同商品规格温朴药材中厚朴酚与和厚朴酚的含量。方法:色谱柱Hypersil BDS C18(250mm×4.6mm,5μm),流动相为甲醇-水(80∶20)。结果:厚朴酚在0.45~230μg/mL,和厚朴酚在0.90~110μg/mL范围内线性关系良好。含量测定结果表明,不同商品规格温朴药材中厚朴酚与和厚朴酚含量存在差异。结论:不同规格温朴的内在质量有明显差异。

OBJECTIVE: To establish a HPLC method for the determination of honokiol and honokiol in different commercial specifications, and to determine the contents of honokiol and honokiol in different commercial specifications of Wenpukao. METHODS: The column was Hypersil BDS C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (80:20). Results: Magnolol had a good linearity in the range of 0.45-230 μg / mL and honokiol in the range of 0.90-110 μg / mL. Content determination results show that different commercial specifications Magnolia officinalis and honokiol content differences. Conclusion: Different specifications of the intrinsic quality of warm Park have significant differences.