生物样品中长春西汀的测定

• 作 者: 姚继红 苏成业 储晓岩
• 出 处: 大连医学院学报
• 发表日期: 1994年01期
• 关键词: 长春西汀 生物样品 高效液相色谱 变异系数 线性关系 日间精密度 标准曲线 血浆样品 特异性 文献报道

本文建立了用反相高效液相色谱法分离并鉴定生物样品中长春西汀的方法。与文献报道方法相比，较为简单，灵敏度、特异性也较高。生物样品标准曲线在５０～８０００ｎｇ／ｍｌ之间有良好线性关系，药物最低检测极限５０ｎｇ／ｍｌ。各生物样品中药物回收率约在９６．１３～１０３．６９％之间，测定结果的变异系数为：日内ＣＶ小于５％，日间ＣＶ小于６％。

In this paper, a method for the separation and identification of vinpocetine in biological samples by reversed-phase high performance liquid chromatography has been established. Compared with the methods reported in the literature, relatively simple, high sensitivity and specificity. The standard curve of biological samples has a good linear relationship between 50 ~ 8000ng / ml, and the lowest limit of detection of drugs is 50ng / ml. The recoveries of various biological samples ranged from 96.13% to 103.69%. The coefficient of variation of the determination results were as follows: the intraday CV was less than 5% and the intraday CV was less than 6%.