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恶性疟原虫体外培养在国内外均已成功,其培养液除含RPMI1640培养粉、HEPES缓冲剂,并用5％NaHCO_3调节pH外,必须添加入血清。因人血清来源困难,且价格昂贵,故寻找代用晶就显得极为重要。我们自1979年8月开始,用兔血清代替人血清连续培养恶性疟原虫400多天,原虫繁殖旺盛,其感染率可高达20％以上,与用人血清同时进行比较无明显差异。兹将实验结果
Plasmodium falciparum in vitro and in vivo have been successful at home and abroad, the medium containing RPMI1640 culture powder, HEPES buffer, and pH with 5% NaHCO_3, it must be added to the serum. Due to the difficulty of human serum sources, and expensive, it is extremely important to find alternative crystals. Since August 1979, we started to continuously culture P. falciparum with rabbit serum instead of human serum for more than 400 days. The protozoan multiplied vigorously and its infection rate was as high as 20% or more. There was no significant difference compared with human serum at the same time. The experimental results will be