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通过乳香提取物(frankincense extract)在大鼠皮肤缺损模型中对周围神经损伤修复的影响,探讨乳香提取物对周围神经损伤的修复作用。将25只SD大鼠造皮肤缺损模型,采用自身对照。一侧为对照组,每日常规消毒;另一侧为试验组,每日涂抹乳香提取物。分别于1、4、7、10、14d5个时间点对创面进行拍照,测量创口面积,并取5只大鼠的创面皮缘备用。用免疫组织化学法检测S-100蛋白的表达及RT-PCR法检测碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、神经生长因子(nerve growth factor,NGF)基因表达量的变化。试验结果表明,创伤愈合率显示,试验组较对照组伤口能更快愈合,且试验组第14天伤口基本愈合。免疫组织化学显示,S-100蛋白试验组较对照组有更多的阳性表达,且在第7天阳性结果更明显。RT-PCR显示,试验组bFGFmRNA表达较对照组有增多趋势,第4和7天与对照组相比差异显著(P<0.01)。试验组NGFmRNA表达较对照组相比,差异均显著(P<0.01)。第7和10天NGFmRNA表达多于对照组。
The effect of frankincense extract on the repair of peripheral nerve injury in the model of skin defect in rats and the repair effect of frankincense extract on peripheral nerve injury were investigated. Twenty-five Sprague-Dawley rats were used to establish skin defect models using self-control. Side of the control group, daily routine disinfection; the other side of the test group, smear mastic extracts daily. The wounds were photographed at 1, 4, 7, 10 and 14 days respectively, and the wound area was measured. The expression of S-100 protein was detected by immunohistochemistry and the expression of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) gene were detected by RT-PCR. The results showed that the rate of wound healing showed that the wounds of the experimental group healed faster than the control group, and the wounds of the experimental group basically healed on the 14th day. Immunohistochemistry showed that the S-100 protein test group had more positive expression than the control group, and the positive result was more obvious on the seventh day. RT-PCR showed that the expression of bFGF mRNA in the experimental group increased more than that of the control group, and there was significant difference (P <0.01) on the 4th and 7th days compared with the control group. Compared with the control group, the expression of NGF mRNA in the experimental group was significantly different (P <0.01). NGF mRNA expression was higher on the 7th and 10th days than that of the control group.