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AIM:To discover the relationship between the genotype andantigen serotype of flagellin C among Saimonella strains.METHODS:Fragment of Saimonella flagellin C in plasmidpLS408 was cloned,sequenced and compared with thecorresponding sequence in other strains.Saimonella strainsincluding two typhi strains,one paratyphoid strain,oneenteritidis and one typhimurium strain were isolated fromoutpatients.Genome DNA was purified respectively fromthese clinical isolates,then the corresponding flagellin Cfragment was amplified by polymerase chain reaction,andthe amplification products were analyzed by agarose gelelectrophoresis.RESULTS:The cloned fragment includes 582 nuacleotidesencoding the variable region and partial conservative regionof Salmonella flagellin C in plasmid pLS408.Withcomparison to the corresponing sequences reportedpreviously,there is only a little difference from other strainswith the same flagellar serotype in both nucleotide andamino acid level.Specific PCR products were amplified inSaimonella strains with flagellar serotype H-1-d includingS.muenchen,typhi and typhimurium,but not in S.paratyphoid C or S.enteritidis strains.CONCLUSION:In this experiment,the specificity ofnucleotide sequence could be found in flagellin C centralvadable regions as it exists in flagellar serotypes inSalmonella.It may be helpful to developing a rapid,sensitive,accurate and PCR-based method to detectSaimonella strains with serotype H-1-d.
AIM: To discover the relationship between the genotype and antigen serotype of flagellin C among Saimonella strains. METHODS: Fragment of Saimonella flagellin C in plasmid pLS408 was cloned, sequenced and compared with the same response in other strains. Saimonella strains containing two typhi strains, one paratyphoid strain, oneenteritidis and one typhimurium strain were isolated from patients. Genome DNA was purified respectively from the same clinical isolates, then the corresponding flagellin Cfragment was amplified by polymerase chain reaction, and the amplification products were analyzed by agarose gelelectrophoresis .RESULTS: The cloned fragment includes 582 nuacleotidesencoding the variable region and partial conservative region of Salmonella flagellin C in plasmid pLS408.Withcomparison to the correspondences sequences reportedpreviously, there is only a little difference from other strains with the same flagellar serotype in both nucleotide and amino acid level. ied inSaimonella strain with flagellar serotype H-1-d including S. muenchen, typhi and typhimurium, but not in S. paratyphoid C or S. enteritidis strain. CONCLUSION: In this experiment, the specificity of nucleotide sequence could be found in flagellin C centralvadable regions as it exists in flagellar serotypes in Salmonella. It may be helpful to develop a rapid, sensitive, accurate and PCR-based method to detectSaimonella strains with serotype H-1-d.