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目的:观察酪氨酸激酶受体B(Tyrosine k inase B,TrkB)在宫颈癌和宫颈上皮内瘤变组织中的表达情况,探讨TrkB与宫颈癌发生发展的关系与临床意义。方法:采用免疫组织化学方法(S-P法)对不同分级30例宫颈癌、89例宫颈上皮内瘤变进行TrkB蛋白表达的检测,并与18例正常宫颈组织中TrkB表达进行比较。结果:TrkB蛋白在正常宫颈组织中大多数呈阴性表达,在宫颈上皮内瘤变和宫颈癌中呈阳性表达,阳性表达分布于细胞质和细胞膜,呈黄色或棕黄色颗粒,弥散性分布,阳性表达率分别为C INⅠ75.0%,C INⅡ83.9%,C INⅢ93.3%,宫颈癌100.0%,其表达强度随病变程度加重而增加,差异有统计学意义(P<0.05)。结论:TrkB的高表达与宫颈癌的发生发展关系密切,检测TrkB有助于预测宫颈癌的发生发展,其蛋白阳性表达可作为宫颈癌及癌前病变诊断的生物学标志。
Objective: To observe the expression of tyrosine kinase B (TrkB) in cervical carcinoma and cervical intraepithelial neoplasia, and to explore the relationship between TrkB and cervical carcinogenesis and its clinical significance. Methods: TrkB protein was detected by immunohistochemistry (S-P method) in 30 cases of cervical cancer and 89 cases of cervical intraepithelial neoplasia, and compared with TrkB expression in 18 cases of normal cervical tissue. Results: TrkB protein was negatively expressed in normal cervical tissue and positive in cervical intraepithelial neoplasia and cervical cancer. The positive expression of TrkB protein was found in cytoplasm and cell membrane, with yellow or brown granules, with diffuse distribution and positive expression The rates were 75.0% for C INⅠ, 83.9% for C INⅡ, 93.3% for CINⅢ, and 100.0% for cervical cancer, respectively. The expression level of CINⅠwas increased with the severity of the pathological changes. The difference was statistically significant (P <0.05). Conclusion: The high expression of TrkB is closely related to the occurrence and development of cervical cancer. Detecting TrkB may be useful for predicting the occurrence and development of cervical cancer. The positive expression of TrkB may serve as a biomarker for the diagnosis of cervical cancer and precancerous lesions.