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目的通过检测氧化亚氮对大鼠耳蜗总RNA产量的影响,探讨氧化亚氮(N_2O)对大鼠内耳损伤的机制。方法将30只健康Wistar大鼠随机等分为3组:A组(对照组,n=10)持续吸入50%O_2 3 h;B组(实验组,n=10)持续吸入50%N_2O+50%O_2 3 h;C组(实验组,n=10)持续吸入50%N_2O+50%O_2 6 h。联用TRIzol和RNeasy法分别抽取3组大鼠耳蜗的总RNA,用分光光度法测总RNA的产量及电泳检测其质量。结果A组大鼠耳蜗得到总RNA 7.69μg;B组大鼠耳蜗得到总RNA 6.51μg,与A组比较减少15%;C组大鼠耳蜗得到总RNA 5.32μg,与A组比较减少31%。A_(260)/A_(280)值分别为2.07、2.04和2.05,提示RNA纯度高,电泳结果提示总RNA无降解。结论长时间吸入N_2O的大鼠耳蜗总RNA较正常大鼠耳蜗总RNA产量降低,提示N_2O干扰耳蜗RNA产量可能是造成耳损害的原因之一。
Objective To investigate the effect of nitrous oxide (N 2 O) on the total RNA production in the rat cochlea and to explore the mechanism of nitrous oxide (N_2O) in the damage of the inner ear. Methods Thirty healthy Wistar rats were equally divided into three groups: group A (control group, n = 10) were continuously inhaled for 50% O 2 3 h; group B (n = 10) % O_2 3 h; Group C (experimental group, n = 10) inhaled 50% N 2 O + 50% O 2 6 h continuously. Total RNA was extracted from the cochlea of rats in each group by TRIzol and RNeasy methods. The total RNA was measured by spectrophotometry and the quality of total RNA was detected by electrophoresis. Results The total RNA was 7.69μg in the cochlea of group A, 6.51μg of total RNA in group B, 15% less than that of group A, and 5.32μg of total RNA in group C, which was 31% less than that of group A. The values of A 260 / A 280 were 2.07, 2.04 and 2.05, respectively, suggesting high purity of RNA and no degradation of total RNA by electrophoresis. Conclusion The total RNA of cochleas of rats with long-term inhalation of N 2 O is lower than that of normal rats, suggesting that N 2 O interfered with the production of cochlear RNA may be one of the causes of ear damage.