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目的:探讨PTEN基因mRNA异常表达及其启动子甲基化与膀胱移行细胞癌的关系。方法:应用逆转录聚合酶链反应(RT-PCR)技术检测膀胱移行细胞癌组织48例和正常对照15例PTEN基因mRNA的表达水平和应用甲基化特异性PCR(MSP)法检测启动子的甲基化。结果:PTEN基因mRNA在膀胱癌患者的表达在48例中有27例,而正常对照全部有表达;正常对照全部未检测到PTEN基因启动子甲基化,而膀胱癌患者启动子甲基化有23例。结论:PTEN基因mRNA表达缺失可能与启动子甲基化有关,在膀胱移行细胞癌的发生、发展及转移过程中起重要作用。
Objective: To investigate the relationship between PTEN mRNA abnormality, promoter methylation and bladder transitional cell carcinoma. Methods: The expression of PTEN mRNA was detected by RT-PCR in 48 cases of bladder transitional cell carcinoma and 15 cases of normal control. Methylation-specific PCR (MSP) was used to detect the expression of promoter Methylation. Results: The expression of PTEN gene mRNA in bladder cancer patients was 27 cases in 48 cases, while the normal control all expressed; PTEN gene promoter methylation was not detected in normal controls, while promoter methylation in bladder cancer patients 23 cases. CONCLUSION: The loss of PTEN gene mRNA expression may be related to promoter methylation and play an important role in the occurrence, development and metastasis of bladder transitional cell carcinoma.