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目的：通过地龙对动物伤口模型的收缩作用的实验，探讨地龙的促伤口愈合作用。方法：在新西兰大白兔背部伤口模型上用药，于伤后５，７，１０，１４ｄ进行肉眼观察，并取肉芽组织，分别做免疫组化ＡＢＣ染色和电镜观察。分别用ｂＦＧＦ和ＰＢＳ做阳性对照和阴性对照。结果：用药组肉芽组织中含大量肌纤维母细胞，合成功能活跃，细胞内肌动蛋白较多，伤口收缩明显，与阴性对照相比有显著性差异，伤口修复较快。结论：地龙可促进肉芽组织中肌纤维母细胞增生，合成功能活跃，分泌伤口收缩的重要物质———肌动蛋白较多，有利于伤口的收缩，对伤口的愈合有促进作用。
OBJECTIVE: To investigate the role of earthworms in promoting wound healing by studying the effects of earthworm on animal wound models. METHODS: New Zealand white rabbits were treated with a wound on the back of the rabbit model. Visual observations were performed at 5, 7, 10, and 14 days after injury. The granulation tissue was harvested and subjected to immunohistochemical ABC staining and electron microscopy. Positive controls and negative controls were used with bFGF and PBS, respectively. RESULTS: The granulation tissue of the medication group contained a large number of myofibroblasts, and the synthesis function was active. There were more intracellular actin and more obvious wound shrinkage. Compared with the negative control, there was a significant difference. The wound repair was faster. Conclusion: Earthworm can promote the proliferation of myofibroblasts in granulation tissue, and its synthesis function is active. The important substance that secretes wound contraction is more actin, which is beneficial to the contraction of wounds and promotes wound healing.