【摘 要】
：
The effects of antisense oligodeoxynucleotide (antisense ODN) to follicle-stimulating hormone receptor (FSHR) and follicle-stimulating hormone (FSH) on the expr
【机 构】
：
Family Planning Research Institute
论文部分内容阅读
The effects of antisense oligodeoxynucleotide (antisense ODN) to follicle-stimulating hormone receptor (FSHR) and follicle-stimulating hormone (FSH) on the expression of proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) were studied in primary culture cells derived from human ovarian mucinous cystadenocarcinoma (OMC). The prlmary OMC cells were cultured with the enzyme digestion method, and the expression of pan Keratin protein and FSHR mRNA was detected for identification of the cells. OMC cells were co-cultured with antisense ODN, nonsense ODN and FSH with different concentrations for 48 h and 72 h. The expression of PCNA and VEGF was detected by using SP immunohistochemistry. Compared with that in the control group, the PCNA and VEGF expression was increased obviously in FSH groups (P＜0.05 or P＜ 0.01), while decreased significantly in antisense ODN groups (P＜0. 05 or P＜0.01) and unchanged in nonsense ODN groups, respectively. Meanwhile, antisense ODN could antagonize the increased expression of PCNA and VEGF caused by FSH significantly (P＜0.01). It was suggested that FSH might promotethe development of OMC to some extent. Antisense ODN could inhibit the proliferative activity of OMC cells and the promoting proliferative activity enhanced by FSH.
其他文献
目的 总结三聚氰胺所致婴幼儿尿路结石的微创外科治疗经验. 方法 回顾性分析36例三聚氰胺所致尿路结石患儿资料.男23例,女13例.年龄8～36个月,平均15个月.患儿均有受三聚氰胺污
目的 探讨MV3黑素瘤细胞接种至人去表皮的真皮(DED)上,体外构建皮肤恶性黑素瘤模型中黑素瘤细胞的侵袭方式.方法 制备含部分基底膜成分的人去表皮的真皮组织(de-epiderrnized
A new approach for the derivation of the principal invariants of the stretch tensor with respect to the right Cauchy Green tensor is presented in this paper. Ac
One concept for immune therapy of cancer involves induction of antigen mimic antibodies to trigger the immune response against tumor cells. Anti-idiotypic antib
Crude decoction, aqueous and ethanolic extracts of two medicinal plants (Psidium guajava and Diospyros mespiliformis), widely used in the central plateau of Bur
We previously reported that v-Fos transformation effector (Fte-1) is a novel CD2 binding protein identified by yeast two hybrid system. In the present study, we
The damages of building structures subjected to multifarious explosions cause huge losses of lives and property.It is the reason why the blast resistance and ex
In order to investigate the expression levels of Pin1 mRNA and protein in cervical cancer and its association with Ki67 and their clinical significance, amplifi
To construct cDNA library of nasopharyngeal carcinoma (NPC) and obtain the NPC associated or specific antigens from it, we used a powerful new method to identif