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采用逆转录 聚合酶链式反应 (RT PCR)方法 ,从鲮肝脏总RNA中扩增出胰岛素样生长因子 I(IGF I)基因 ,克隆至质粒pUCm -T。测定该基因序列 ,推导其编码的蛋白质序列。克隆的鲮IGF IcDNA编码序列包括信号肽、B、C、A、D和E 6个区域 ,共 16 1个氨基酸残基。与鲤IGF I比较 ,信号肽由 4 4个氨基酸残基组成比鲤少 17个 ,成熟肽核苷酸序列和氨基酸序列的同源性分别为 95 .2 %和 10 0 %。E区域分析结果表明 ,克隆的鲮IGF I序列属于IGF IEa 2亚型
The mRNA of insulin-like growth factor I (IGF-I) was amplified from the total RNA of the liver by reverse transcription-polymerase chain reaction (RT PCR) and cloned into plasmid pUCm-T. The gene sequence is determined and the protein sequence it encodes is deduced. The cloned 鲮 IGF IcDNA coding sequence includes a signal peptide, B, C, A, D and E 6 regions, totaling 161 amino acid residues. Compared with the common carp IGF I, the signal peptide consisted of 44 amino acid residues less than the common carp, and the homologies of the nucleotide and amino acid sequences of the mature peptide were 95.2% and 100.0%, respectively. E-region analysis showed that the cloned IGF I sequence belongs to the IGF IEa 2 subtype