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目的利用针对人端粒酶RNA(hTR)的反义寡核苷酸(ASODN)和正义寡核苷酸(NODN)作用于人肝癌细胞SMMC-7721,比较ASODN和NODN作用后细胞培养液蛋白的变化。方法利用SELDI技术检测差异蛋白的表达变化。对照组为未加任何处理因素的SMMC-7721细胞。结果SELDI技术检测发现,ASODN作用组有40个差异蛋白分子低表达,3个差异蛋白分子高表达。NODN作用组有37个差异蛋白分子低表达,6个差异蛋白分子高表达。所有差异蛋白分子量均小于10 000 Da。ASODN作用组中有3个低表达蛋白在NODN作用组高表达,分子量分别为3 011.99 Da、3 048.28 Da和3 248.75 Da。结论ASODN和NODN作用SMMC-7721细胞后细胞培养液中所表达的差异蛋白十分相似。
OBJECTIVE: To investigate the effects of ASODN and NODN on the proliferation of human hepatocellular carcinoma cell line SMMC-7721 after antisense oligonucleotide (ASODN) and sense oligodeoxynucleotide (NODN) targeting human telomerase RNA (hTR) Variety. Methods SELDI technique was used to detect the expression of differential proteins. The control group was SMMC-7721 cells without any treatment factors. Results The results of SELDI assay showed that 40 differentially expressed protein molecules were low expressed in ASODN group and 3 differentially expressed protein molecules were highly expressed. In the NODN group, 37 differentially expressed protein molecules were low and 6 differentially expressed protein molecules were highly expressed. All differential proteins have molecular weights less than 10 000 Da. Three of the low expression proteins in the ASODN-treated group were highly expressed in the NODN group with molecular weights of 3 011.99 Da, 3 048.28 Da and 3 248.75 Da, respectively. Conclusion The expression of differential proteins in SMMC-7721 cells after ASODN and NODN treatment are very similar.