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本文采用硅胶HF_(254)薄层,以氯仿-95％乙醇-乙酸-水(54:27:9:4)为展开剂,直接分离制剂中的维生素B_2、C和烟酰胺等水溶性维生素。用石油醚提取制剂中的脂溶性成分后,再以石油醚-乙醚(4:1)为展开剂分离维生素A、D、和E。最后于CS-910型双波长薄层扫描仪上,采用反射式锯齿形扫描,分别对维生素A、B_2、C和烟酰胺进行扫描测定。维生素Cλ_s=250nm,λ_R=400nm;烟酰胺λ_s=260nm,λ_R=400nm;维生素B_2λ_s=444nm,λ_R=700nm;维生素Aλ_s=300nm,λ_R=400nm。线性关系和精度都比较满意。回收率:维生素C 99.2％,维生素B_2100.4％,维生素A 99.9％,烟酰胺99.4％;变异系数分别为:2.1％,1.1％,1.9％和1.2%。
In this work, vitamin B 2, C, nicotinamide and other water-soluble vitamins were separated directly from the thin layer of silica gel (HF_ (254)) with chloroform -95% ethanol-acetic acid-water (54: 27: 9: 4) After extracting fat-soluble components in petroleum ether, vitamins A, D and E were separated by petroleum ether-ether (4: 1). At the end of this study, a reflective zigzag scanning was performed on the CS-910 dual-wavelength thin-layer scanner to measure the vitamin A, B_2, C and nicotinamide respectively. Vitamin C 2_s = 250 nm and λ R = 400 nm; Nicotinamide λ_s = 260 nm and λ_R = 400 nm; Vitamin B 2λ_s = 444 nm and λ_R = 700 nm; Vitamin Aλ_s = 300 nm and λ_R = 400 nm. Linear relationship and accuracy are more satisfied. The recoveries were 99.2% for vitamin C, 2100.4% for vitamin B, 99.9% for vitamin A and 99.4% for nicotinamide. The coefficients of variation were 2.1%, 1.1%, 1.9% and 1.2%, respectively.